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Mouse Anti-CEMIP Recombinant Antibody (3C12) (CBMAB-A4683-LY)

The product is antibody recognizes KIAA1199. The antibody 3C12 immunoassay techniques such as: WB, ELISA.
See all CEMIP antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human
Clone
3C12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
KIAA1199 (NP_061159.1, 880 a.a. ~ 979 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cell Migration Inducing Hyaluronan Binding Protein
Entrez Gene ID
UniProt ID
Alternative Names
TMEM2L
Function
Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.
Biological Process
Hyaluronan biosynthetic process Source: Reactome
Hyaluronan catabolic process Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of peptidyl-threonine phosphorylation Source: UniProtKB
Positive regulation of protein kinase C activity Source: UniProtKB
Positive regulation of protein targeting to membrane Source: UniProtKB
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Sensory perception of sound Source: UniProtKB
Cellular Location
Secreted; Nucleus; Cytoplasm; Cell membrane; Endoplasmic reticulum; Clathrin-coated pit. Retained in the endoplasmic reticulum (ER) in a HSPA5/BIP-dependent manner. Colocalized with clathrin heavy chain/CLTC in clathrin-coated vesicles. Strongly detected in the cytoplasm of breast carcinoma cells, whereas poorly detected in adjacent normal epithelial cells, stromal cells, or benign breast tissues. Localized in the nucleus and cytoplasm of colon adenocarcinomas.
PTM
N-glycosylated; glycosylation is not necessary for HA-binding.

Chen, Y., Zhou, H., Jiang, W. J., Wang, J. F., Tian, Y., Jiang, Y., & Xia, B. R. (2022). The role of CEMIP in tumors: An update based on cellular and molecular insights. Biomedicine & Pharmacotherapy, 146, 112504.

Chen, Y., Li, L., & Zhang, J. (2021). Cell migration inducing hyaluronidase 1 (CEMIP) activates STAT3 pathway to facilitate cell proliferation and migration in breast cancer. Journal of Receptors and Signal Transduction, 41(2), 145-152.

Song, M., Liu, J., Zheng, X., Zhou, X., Feng, Z., & Hu, W. (2021). MiR-148a-3p targets CEMIP to suppress the genesis of gastric cancer cells. Biochemical and Biophysical Research Communications, 575, 42-49.

Sato, S., Mizutani, Y., Yoshino, Y., Masuda, M., Miyazaki, M., Hara, H., & Inoue, S. (2021). Pro-inflammatory cytokines suppress HYBID (hyaluronan (HA)-binding protein involved in HA depolymerization/KIAA1199/CEMIP)-mediated HA metabolism in human skin fibroblasts. Biochemical and Biophysical Research Communications, 539, 77-82.

Ohtsuki, T., Hatipoglu, O. F., Asano, K., Inagaki, J., Nishida, K., & Hirohata, S. (2020). Induction of CEMIP in chondrocytes by inflammatory cytokines: underlying mechanisms and potential involvement in osteoarthritis. International journal of molecular sciences, 21(9), 3140.

Dokoshi, T., Zhang, L. J., Li, F., Nakatsuji, T., Butcher, A., Yoshida, H., ... & Gallo, R. L. (2020). Hyaluronan degradation by Cemip regulates host defense against staphylococcus aureus skin infection. Cell reports, 30(1), 61-68.

Hu, R., & Lu, Z. (2020). Long non‑coding RNA HCP5 promotes prostate cancer cell proliferation by acting as the sponge of miR‑4656 to modulate CEMIP expression. Oncology reports, 43(1), 328-336.

Li, L., Pan, Y., Mo, X., Wei, T., Song, J., Luo, M., ... & Yang, J. (2020). A novel metastatic promoter CEMIP and its downstream molecular targets and signaling pathway of cellular migration and invasion in SCLC cells based on proteome analysis. Journal of Cancer Research and Clinical Oncology, 146(10), 2519-2534.

Liang, G., Fang, X., Yang, Y., & Song, Y. (2018). Knockdown of CEMIP suppresses proliferation and induces apoptosis in colorectal cancer cells: downregulation of GRP78 and attenuation of unfolded protein response. Biochemistry and Cell Biology, 96(3), 332-341.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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