Mouse Anti-CERS1 Recombinant Antibody (2E8) (CBMAB-A4862-LY)

The product is antibody recognizes LASS1. The antibody 2E8 immunoassay techniques such as: WB, ELISA.
See all CERS1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2E8

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

LASS1 (NP_067090.1, 301 a.a. ~ 350 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Ceramide Synthase 1

Introduction

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. Members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in yeast suggest that the encoded protein is involved in aging. This protein is transcribed from a monocistronic mRNA as well as a bicistronic mRNA, which also encodes growth differentiation factor 1. [provided by RefSeq]

Entrez Gene ID

10715

UniProt ID

P27544

Alternative Names

CerS1; LAG1; MGC90349; UOG1

Function

Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward stearoyl-CoA (octadecanoyl-CoA; C18:0-CoA).

Biological Process

Cellular response to dithiothreitol Source: UniProtKB
Cellular response to drug Source: UniProtKB
Cellular response to mycotoxin Source: UniProtKB
Cellular response to UV-A Source: UniProtKB
Ceramide biosynthetic process Source: UniProtKB
Negative regulation of telomerase activity Source: UniProtKB
Sphingolipid biosynthetic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane

Involvement in disease

Epilepsy, progressive myoclonic 8 (EPM8): A form of progressive myoclonic epilepsy, a clinically and genetically heterogeneous group of disorders defined by the combination of action and reflex myoclonus, other types of epileptic seizures, and progressive neurodegeneration and neurocognitive impairment. EPM8 is an autosomal recessive form characterized by myoclonus, generalized tonic-clonic seizures and moderate to severe cognitive impairment.

PTM

Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.

References

Chen, W., Wu, C., Chen, Y., Guo, Y., Qiu, L., Liu, Z., ... & Li, L. (2021). Downregulation of ceramide synthase 1 promotes oral cancer through endoplasmic reticulum stress. International journal of oral science, 13(1), 1-9.

Tosetti, B., Brodesser, S., Brunn, A., Deckert, M., Blüher, M., Doehner, W., ... & Krönke, M. (2020). A tissue‐specific screen of ceramide expression in aged mice identifies ceramide synthase‐1 and ceramide synthase‐5 as potential regulators of fiber size and strength in skeletal muscle. Aging cell, 19(1), e13049.

Turpin-Nolan, S. M., Hammerschmidt, P., Chen, W., Jais, A., Timper, K., Awazawa, M., ... & Brüning, J. C. (2019). CerS1-derived C18: 0 ceramide in skeletal muscle promotes obesity-induced insulin resistance. Cell Reports, 26(1), 1-10.

Turner, N., Lim, X. Y., Toop, H. D., Osborne, B., Brandon, A. E., Taylor, E. N., ... & Don, A. S. (2018). A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism. Nature communications, 9(1), 1-14.

Wang, Z., Wen, L., Zhu, F., Wang, Y., Xie, Q., Chen, Z., & Li, Y. (2017). Overexpression of ceramide synthase 1 increases C18-ceramide and leads to lethal autophagy in human glioma. Oncotarget, 8(61), 104022.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-CERS1 Recombinant Antibody (CBYJL-082)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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