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Mouse Anti-MAP2K5 Recombinant Antibody (V2-615348) (CBMAB-A5196-LY)

The product is antibody recognizes MAP2K5. The antibody 2C2 immunoassay techniques such as: WB, ELISA.
See all MAP2K5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-615348
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
MAP2K5 (AAH08838, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
mitogen-activated protein kinase kinase 5
Introduction
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq]
Entrez Gene ID
UniProt ID
Alternative Names
HsT17454; MAPKK5; MEK5; PRKMK5
Function
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
Biological Process
Cellular response to growth factor stimulusBy SimilarityISS:BHF-UCL
Cellular response to laminar fluid shear stressManual Assertion Based On ExperimentTAS:BHF-UCL
ERK5 cascadeIEA:Ensembl
Heart developmentIEA:Ensembl
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of cell migration involved in sprouting angiogenesisBy SimilarityISS:BHF-UCL
Negative regulation of chemokine (C-X-C motif) ligand 2 productionBy SimilarityISS:BHF-UCL
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic processBy SimilarityISS:BHF-UCL
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligandBy SimilarityISS:BHF-UCL
Negative regulation of heterotypic cell-cell adhesionBy SimilarityISS:BHF-UCL
Negative regulation of interleukin-8 productionBy SimilarityISS:BHF-UCL
Negative regulation of NF-kappaB transcription factor activityBy SimilarityISS:BHF-UCL
Negative regulation of response to cytokine stimulusBy SimilarityISS:BHF-UCL
Negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Positive regulation of cell growthIEA:Ensembl
Positive regulation of epithelial cell proliferationIEA:Ensembl
Positive regulation of MAP kinase activityBy SimilarityISS:BHF-UCL
Positive regulation of protein metabolic processISS:BHF-UCL
Positive regulation of transcription by RNA polymerase IIISS:BHF-UCL
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytosol
Nucleus
Spindle
PTM
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
(Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Qiu, M., Lin, Q., Liu, Y., Chen, P., Zhou, Y., Jiang, Y., ... & Yu, H. (2023). Potentially functional genetic variants in RPS6KA4 and MAP2K5 in the MAPK signaling pathway predict HBV‐related hepatocellular carcinoma survival. Molecular Carcinogenesis.

Lu, C., Wang, H. J., Song, J. Y., Wang, S., Li, X. Y., Huang, T., & Wang, H. (2022). Fine Mapping of the MAP2K5 Region Identified rs7175517 as a Causal Variant Related to BMI in China and the United Kingdom Populations. Frontiers in Genetics, 13, 838685.

Huang, Y., Wang, P., Morales, R., Luo, Q., & Ma, J. (2021). Map2k5-deficient mice manifest phenotypes and pathological changes of dopamine deficiency in the central nervous system. Frontiers in Aging Neuroscience, 13, 651638.

Ye, F., Gao, H., Xiao, L., Ding, H., Huang, Z., Jiang, Y., ... & Bu, H. (2019). Authors' reply to: Absence of the MAP2K5 germline variants c. G961A and c. T1100C in a wide series of familial non-medullary thyroid carcinoma Italian families. International Journal of Cancer 2019; in press. International journal of cancer, 145(2), 601-602.

Cirello, V., Colombo, C., Persani, L., & Fugazzola, L. (2019). Absence of the MAP2K5 germline variants c. G961A and c. T1100C in a wide series of familial nonmedullary thyroid carcinoma Italian families. International journal of cancer, 145(2), 600-600.

Ye, F., Gao, H., Xiao, L., Zuo, Z., Liu, Y., Zhao, Q., ... & Bu, H. (2019). Whole exome and target sequencing identifies MAP2K5 as novel susceptibility gene for familial non‐medullary thyroid carcinoma. International journal of cancer, 144(6), 1321-1330.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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