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Rat Anti-PRIM1 Recombinant Antibody (8G10) (CBMAB-CP1831-LY)

The product is antibody recognizes p48 Primase. The antibody 8G10 immunoassay techniques such as: WB.
See all PRIM1 antibodies

Summary

Host Animal
Rat
Specificity
Human, Mouse, Rat, Monkey
Clone
8G10
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with full-length recombinant human p48 primase.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DNA primase subunit 1
Entrez Gene ID
Human5557
Mouse19075
Rat246327
Monkey710253
UniProt ID
HumanP49642
MouseP20664
RatF1LNK6
MonkeyG7N7D9
Function
Catalytic subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex - primosome/replisome) which play an essential role in the initiation of DNA synthesis (PubMed:9268648, PubMed:9705292, PubMed:17893144, PubMed:24043831, PubMed:26975377, PubMed:25550159, PubMed:31479243).
During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity).
The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (PubMed:17893144).
These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).
In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (PubMed:17893144).
Synthesizes 9-mer RNA primers (also known as the 'unit length' RNA primers). Incorporates only ribonucleotides in the presence of ribo- and deoxy-nucleotide triphosphates (rNTPs, dNTPs) (PubMed:26975377).
Requires template thymine or cytidine to start the RNA primer synthesis, with an adenine or guanine at its 5'-end (PubMed:25550159, PubMed:26975377).
Binds single stranded DNA (By similarity).
Biological Process
DNA replication initiationManual Assertion Based On ExperimentIDA:ComplexPortal
DNA replication, synthesis of RNA primerManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
alpha DNA polymerase:primase complex
membrane
nucleoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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