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Rabbit Anti-Phospho-GRB10 (Ser476) Recombinant Antibody (D4E6) (CBMAB-CP0870-LY)

The product is antibody recognizes Phospho-GRB10 (Ser476). The antibody D4E6 immunoassay techniques such as: WB,IP.
See all Phospho-GRB10 (Ser476) antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D4E6
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser476 of human GRB10 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Growth Factor Receptor Bound Protein 10
Introduction
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]
Entrez Gene ID
Human2887
Mouse14783
Rat498416
UniProt ID
HumanQ13322
MouseQ60760
RatP0CE43
Alternative Names
Growth Factor Receptor Bound Protein 10; Growth Factor Receptor-Bound Protein 10; Insulin Receptor-Binding Protein Grb-IR; GRB10 Adapter Protein; Maternally Expressed Gene 1; GRB10 Adaptor Protein; KIAA0207;
Function
Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
Biological Process
ERK1 and ERK2 cascade Source: Ensembl
Gene expression Source: Ensembl
Insulin-like growth factor receptor signaling pathway Source: Ensembl
Insulin receptor signaling pathway Source: BHF-UCL
Negative regulation of glucose import Source: BHF-UCL
Negative regulation of glycogen biosynthetic process Source: BHF-UCL
Negative regulation of insulin receptor signaling pathway Source: BHF-UCL
Negative regulation of phosphorylation Source: Ensembl
Negative regulation of Wnt signaling pathway Source: UniProtKB
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of phosphorylation Source: BHF-UCL
Positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
Response to insulin Source: BHF-UCL
Vascular associated smooth muscle cell migration Source: Ensembl
Cellular Location
Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity).
PTM
Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation (By similarity). Phosphorylated at Tyr-67 by TEC.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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