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Mouse Anti-PNKP Recombinant Antibody (B-4) (CBMAB-P2251-YC)

Provided herein is a Mouse monoclonal antibody against Human Polynucleotide Kinase 3'-Phosphatase. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all PNKP antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
B-4
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Polynucleotide Kinase 3'-Phosphatase
Introduction
PNKP represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Entrez Gene ID
11284
UniProt ID
Q96T60
Alternative Names
AOA4; EIEE10; MCSZ; PNK
Function
Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed:10446192, PubMed:10446193, PubMed:15385968, PubMed:20852255, PubMed:28453785).
Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed:10446192, PubMed:10446193).
Biological Process
Base-excision repair, gap-fillingTAS:Reactome
DNA ligation involved in DNA repairManual Assertion Based On ExperimentIDA:UniProtKB
DNA repairManual Assertion Based On ExperimentIGI:UniProtKB
DNA-templated DNA replication1 PublicationNAS:UniProtKB
Double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein ADP-ribosylationManual Assertion Based On ExperimentIMP:UniProtKB
Nucleotide phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Nucleotide-excision repair, DNA damage removal1 PublicationNAS:UniProtKB
Positive regulation of double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIMP:CACAO
Positive regulation of telomerase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere cappingManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere maintenance via telomeraseManual Assertion Based On ExperimentIMP:BHF-UCL
Response to oxidative stressManual Assertion Based On ExperimentIDA:UniProtKB
Response to radiationManual Assertion Based On ExperimentTAS:UniProtKB
Cellular Location
Nucleus
Chromosome
Localizes to site of double-strand breaks.
Involvement in disease
Microcephaly, seizures, and developmental delay (MCSZ):
An autosomal recessive neurodevelopmental disorder characterized by infantile-onset seizures, microcephaly, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
Ataxia-oculomotor apraxia 4 (AOA4):
An autosomal recessive disease characterized by cerebellar ataxia, oculomotor apraxia, areflexia and peripheral neuropathy.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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