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Recombinant Mouse Anti-Respiratory Syncytial Virus (RSV) Fusion Protein Antibody (V2-624510) (CBMAB-MD281-LY)

The product is antibody recognizes RSV. The antibody CB1878 immunoassay techniques such as: ELISA.
See all Respiratory Syncytial Virus (RSV) Fusion Protein antibodies

Summary

Host Animal
Mouse
Specificity
RSV
Clone
V2-624510
Antibody Isotype
IgG2a
Application
ELISA

Basic Information

Immunogen
Purified native RSV protein, Long strain
Specificity
RSV
Antibody Isotype
IgG2a
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.05% Sodium Azide
Purity
> 95% Purity determined by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Respiratory Syncytial Virus (RSV) Fusion Protein
Function
Fusion glycoprotein F0
Inactive precursor that is cleaved at two sites by a furin-like protease to give rise to the mature F1 and F2 fusion glycoproteins.
Fusion glycoprotein F1
Class I viral fusion protein (PubMed:23618766).
Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state (PubMed:23618766).
During viral and plasma cell membrane fusion, the coiled coil regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain (PubMed:23618766, PubMed:19966279).
The formation of this structure appears to drive apposition and subsequent fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm (PubMed:23593008, PubMed:23618766).
This fusion is pH independent and occurs at the plasma or endosomal membrane (Probable). The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate (PubMed:10864656).
F protein is involved in the entry into the host cell through the interaction with host IGFR1 (PubMed:32494007).
This interaction activates PRKCZ/PKCzeta that recruits host NCL/nucleolin to the apical cell surface where it can bind fusion glycoprotein F1 (PubMed:32494007, PubMed:21841784).
Later in infection, F protein expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis (PubMed:10438814).
F protein may trigger p53-dependent apoptosis (PubMed:18216092).
Fusion glycoprotein F2
Major determinant of the species specificity of RSV infection (PubMed:12663767).
The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate (PubMed:10864656).
F protein is involved in the entry into the host cell through the interaction with host IGFR1 (PubMed:32494007).
This interaction activates PRKCZ/PKCzeta that recruits host NCL/nucleolin to the apical cell surface where it can bind fusion glycoprotein F1 (PubMed:32494007).
Later in infection, F protein expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis (PubMed:10438814).
F protein seems to trigger p53-dependent apoptosis (PubMed:18216092).
Biological Process
Biological Process entry receptor-mediated virion attachment to host cellIEA:UniProtKB-KW
Biological Process fusion of virus membrane with host plasma membraneIEA:UniProtKB-KW
Biological Process positive regulation of syncytium formation by virusIEA:UniProtKB-KW
Biological Process viral entry into host cellIEA:UniProtKB-KW
Cellular Location
Fusion glycoprotein F0
Host Golgi apparatus membrane
Fusion glycoprotein F1
Virion membrane
Host cell membrane
Localized at the host apical membrane.
Fusion glycoprotein F2
Virion membrane
Host cell membrane
Localized at the host apical membrane.
Topology
Extracellular: 26-524
Helical: 525-550
Cytoplasmic: 551-574
PTM
Fusion glycoprotein F0
The F glycoprotein is synthesized as a F0 inactive precursor that is heavily N-glycosylated and processed at two sites by a host furin-like protease probably in the Golgi (PubMed:11493675, PubMed:11369882, PubMed:23593008, PubMed:11418598).
The cleavage site between p27 and F1 may occur after endocytosis to yield the mature F1 and F2 proteins (Probable). Both cleavages are required for membrane fusion and p27 is released from the processed protein (PubMed:11493675, PubMed:23593008, PubMed:12127793).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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