Rabbit Anti-TMED10 Monoclonal Antibody (EPR9037(B)) (CBMAB-1644-YC)

Rabbit

Human, Mouse, Rat

EPR9037(B)

IgG

WB

Synthetic peptide corresponding to residues in Human TMP21.

Human, Mouse, Rat

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Transmembrane P24 Trafficking Protein 10

TMED10 is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. TMED10 is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. TMED10 is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in TMED10 have been associated with early-onset familial Alzheimer's disease.

p23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA)

Cargo receptor involved in protein vesicular trafficking and quality control in the endoplasmic reticulum (ER) and Golgi (PubMed:10052452, PubMed:11726511, PubMed:16641999, PubMed:17288597, PubMed:19296914, PubMed:20427317, PubMed:21219331, PubMed:27569046).
The p24 protein family is a group of transmembrane proteins that bind coat protein complex I/COPI and coat protein complex II/COPII involved in vesicular trafficking between the membranes (PubMed:10052452).
Acts at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and involved in vesicle coat formation at the cytoplasmic side (PubMed:20427317, PubMed:27569046).
Mainly functions in the early secretory pathway and cycles between the ER, ER-Golgi intermediate compartment (ERGIC) and Golgi, mediating cargo transport through COPI and COPII-coated vesicles (PubMed:10052452, PubMed:10852829, PubMed:12237308).
In COPII vesicle-mediated anterograde transport, involved in the transport of GPI-anchored proteins by acting together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER (PubMed:20427317, PubMed:27569046).
Recognizes GPI anchors structural remodeled in the ER by the GPI inositol-deacylase/PGAP1 and the metallophosphoesterase MPPE1/PGAP5 (By similarity).
In COPI vesicle-mediated retrograde transport, involved in the biogenesis of COPI vesicles and vesicle coat recruitment (PubMed:11726511).
Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent from the modulation of gamma-secretase activity) (PubMed:17288597).
Involved in the KDELR2-mediated retrograde transport of the toxin A subunit (CTX-A-K63)together with COPI and the COOH terminus of KDELR2 (By similarity).
On Golgi membranes, acts as primary receptor for ARF1-GDP, a GTP-binding protein involved in COPI-vesicle formation (PubMed:11726511).
Increases coatomer-dependent GTPase-activating activity of ARFGAP2 which mediates the hydrolysis of ARF1-bound GTP and therefore modulates protein trafficking from the Golgi apparatus (PubMed:19296914).
Involved in the exocytic trafficking of G protein-coupled receptors F2LR1/PAR2 (trypsin and tryspin-like enzyme receptor), OPRM1 (opioid receptor) and P2RY4 (UTD and UDP receptor) from the Golgi to the plasma membrane, thus contributing to receptor resensitization (PubMed:21219331).
In addition to its cargo receptor activity, may also act as a protein channel after oligomerization, facilitating the post-translational entry of leaderless cytoplasmic cargo into the ERGIC (PubMed:32272059).
Involved in the translocation into ERGIC, the vesicle entry and the secretion of leaderless cargos (lacking the secretion signal sequence), including the mature form of interleukin 1/IL-1 family members, the alpha-crystallin B chain HSPB5, the carbohydrate-binding proteins galectin-1/LGALS1 and galectin-3/LGALS3, the microtubule-associated protein Tau/MAPT, and the annexin A1/ANXA1; the translocation process is dependent on cargo protein unfolding and enhanced by chaperones HSP90AB1 and HSP90B1/GRP9 (PubMed:32272059).
Could also associates with the presenilin-dependent gamma-secretase complex in order to regulate gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40/Abeta40 (PubMed:16641999).

Biological Process COPI coating of Golgi vesicleSource:UniProtKB1 Publication
Biological Process COPI-coated vesicle buddingSource:UniProtKB1 Publication
Biological Process COPII vesicle coatingSource:UniProtKB1 Publication
Biological Process cytosol to ERGIC protein transportSource:UniProtKB1 Publication
Biological Process endoplasmic reticulum to Golgi vesicle-mediated transportSource:GO_Central1 Publication
Biological Process Golgi organizationSource:GO_Central1 Publication
Biological Process intracellular protein transportSource:UniProtKB1 Publication
Biological Process negative regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic processSource:Ensembl
Biological Process positive regulation of interleukin-1 productionSource:UniProtKB1 Publication
Biological Process positive regulation of protein secretionSource:UniProtKB1 Publication
Biological Process protein localization to ERGICSource:UniProtKB1 Publication
Biological Process regulated exocytosisSource:HGNC-UCL
Biological Process regulation of amyloid-beta formationSource:UniProtKB1 Publication
Biological Process retrograde vesicle-mediated transport, Golgi to endoplasmic reticulumSource:UniProtKB
Biological Process vesicle cargo loadingSource:UniProtKB1 Publication
Biological Process vesicle targeting, to, from or within GolgiSource:HGNC-UCL

Endoplasmic reticulum membrane
Endoplasmic reticulum-Golgi intermediate compartment membrane Analysis
Golgi apparatus membrane
Golgi apparatus, cis-Golgi network membrane
Golgi apparatus, trans-Golgi network membrane
Cytoplasmic vesicle, secretory vesicle membrane
Cell membrane
Melanosome
Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Lumenal: 32-185
Helical: 186-206
Cytoplasmic: 207-219