Human Recombinant OPN protein, His Tag (V2LY-0526-LY5887)

Name: Human Recombinant OPN protein, His Tag
SKU: V2LY-0526-LY5887
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant OPN protein, His Tag consist of Amino Acid: 1-314 and predicts a molecular mass of 35 kDa.

Molecule Mass

35 kDa

Sequence

Amino Acid: 1-314

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>80% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MES, NaCl, Glycerol, Tween20, Trehalose

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Secreted Phosphoprotein 1

Function

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Biological Process

Biological Process androgen catabolic processManual Assertion Based On ExperimentIDA:CAFA
Biological Process biomineral tissue developmentIEA:UniProtKB-KW
Biological Process cell adhesionManual Assertion Based On ExperimentIDA:ARUK-UCL
Biological Process cellular response to testosterone stimulusManual Assertion Based On ExperimentIDA:CAFA
Biological Process decidualizationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process embryo implantationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process inflammatory responseIEA:Ensembl
Biological Process negative regulation of collateral sprouting of intact axon in response to injuryIEA:Ensembl
Biological Process osteoblast differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of bone resorptionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:CAFA
Biological Process positive regulation of estradiol secretionManual Assertion Based On ExperimentIDA:CAFA
Biological Process response to macrophage colony-stimulating factorIEA:Ensembl
Biological Process response to steroid hormoneIEA:Ensembl
Biological Process response to vitamin DManual Assertion Based On ExperimentIDA:BHF-UCL

Cellular Location

Secreted

PTM

Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties.
Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039).
The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity).
O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant OPN protein, His Tag-1 (CAT#: V2LY-0526-LY5888)

SPP1 Matched Antibody Pair (1134) (CAT#: APMAB-1134LY)

SPP1 Matched Antibody Pair (1135) (CAT#: APMAB-1135LY)

SPP1 Matched Antibody Pair (1136) (CAT#: APMAB-1136LY)

SPP1 Matched Antibody Pair (1137) (CAT#: APMAB-1137LY)

SPP1 Matched Antibody Pair (1138) (CAT#: APMAB-1138LY)

SPP1 Matched Antibody Pair (1139) (CAT#: APMAB-1139LY)

Antibody Pairs

SPP1 Matched Antibody Pair (1134) (CAT#: APMAB-1134LY)

SPP1 Matched Antibody Pair (1135) (CAT#: APMAB-1135LY)

SPP1 Matched Antibody Pair (1136) (CAT#: APMAB-1136LY)

SPP1 Matched Antibody Pair (1137) (CAT#: APMAB-1137LY)

SPP1 Matched Antibody Pair (1138) (CAT#: APMAB-1138LY)

SPP1 Matched Antibody Pair (1139) (CAT#: APMAB-1139LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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