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Mouse Anti-MAPK10 Recombinant Antibody (3C9-B0-D5) (CBMAB-Z0370-LY)

The product is antibody recognizes JNK3. The antibody 3C9-B0-D5 immunoassay techniques such as: WB, ICC.
See all MAPK10 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
3C9-B0-D5
Antibody Isotype
IgG1
Application
WB, ICC

Basic Information

Immunogen
Purified recombinant fragment of human MAPK10 (aa28-233) expressed in E. Coli.
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
mitogen-activated protein kinase 10
Entrez Gene ID
Human5602
Mouse26414
UniProt ID
HumanP53779
MouseQ61831
Alternative Names
JNK3; JNK3A; PRKM10; p54bSAPK
Function
Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692).
Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).
Biological Process
Cellular senescenceTAS:Reactome
Fc-epsilon receptor signaling pathwayTAS:Reactome
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
JNK cascadeBy SimilarityISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of circadian rhythmISS:UniProtKB
Regulation of DNA-binding transcription factor activityTAS:Reactome
Response to light stimulusISS:UniProtKB
Rhythmic processIEA:UniProtKB-KW
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Membrane
Nucleus
Mitochondrion
Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity).
Involvement in disease
A chromosomal aberration involving MAPK10 has been found in a single patient with pharmacoresistant epileptic encephalopathy. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
PTM
Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
Palmitoylation regulates subcellular location and axonal development.

Deng, Y. W., Liu, F., Li, Z. T., Gao, J. H., Zhao, Y., Yang, X. L., & Xia, Y. L. (2022). Hyperglycemia promotes myocardial dysfunction via the ERS-MAPK10 signaling pathway in db/db mice. Laboratory Investigation, 102(11), 1192-1202.

Zhang, Y., Long, Y., Liu, Y., Yang, M., Wang, L., Liu, X., ... & Luo, Y. (2022). MAPK5 and MAPK10 overexpression influences strawberry fruit ripening, antioxidant capacity and resistance to Botrytis cinerea. Planta, 255(1), 19.

Liu, F., Deng, Y., Zhao, Y., Li, Z., Gao, J., Zhang, Y., ... & Xia, Y. (2022). Time series RNA-seq analysis identifies MAPK10 as a critical gene in diabetes mellitus-induced atrial fibrillation in mice. Journal of Molecular and Cellular Cardiology, 168, 70-82.

Gao, Y., Wang, Y., Wang, X., Zhao, C., Wang, F., Du, J., ... & Zhang, J. (2021). miR-335-5p suppresses gastric cancer progression by targeting MAPK10. Cancer Cell International, 21(1), 1-12.

Li, H., Li, Y., Zhang, Y., Tan, B., Huang, T., Xiong, J., ... & Fu, L. (2021). MAPK10 Expression as a Prognostic Marker of the Immunosuppressive Tumor Microenvironment in Human Hepatocellular Carcinoma. Frontiers in Oncology, 11, 687371.

Wu, X., Li, X., & Xu, G. (2020). Propofol suppresses the progression of non‑small cell lung cancer via downregulation of the miR‑21‑5p/MAPK10 axis. Oncology Reports, 44(2), 487-498.

Sun, R., Xiang, T., Tang, J., Peng, W., Luo, J., Li, L., ... & Tao, Q. (2020). 19q13 KRAB zinc-finger protein ZNF471 activates MAPK10/JNK3 signaling but is frequently silenced by promoter CpG methylation in esophageal cancer. Theranostics, 10(5), 2243.

Zhang, L., Li, H., Yuan, M., Li, M., & Zhang, S. (2019). Cervical cancer cells-secreted exosomal microRNA-221-3p promotes invasion, migration and angiogenesis of microvascular endothelial cells in cervical cancer by down-regulating MAPK10 expression. Cancer management and research, 10307-10319.

Xie, Y., Liu, Y., Fan, X., Zhang, L., Li, Q., Li, S., ... & Xiao, Y. (2019). MicroRNA-21 promotes progression of breast cancer via inhibition of mitogen-activated protein kinase10 (MAPK10). Bioscience reports, BSR20181000-BSR20181000.

Liu, J., & Li, Y. (2019). Upregulation of MAPK10, TUBB2B and RASL11B may contribute to the development of neuroblastoma. Molecular medicine reports, 20(4), 3475-3486.

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For research use only. Not intended for any clinical use.

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