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Mouse Anti-BCL2L1 Recombinant Antibody (H5) (CBMAB-1025CQ)
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Mouse Anti-ASTN1 Recombinant Antibody (H-9) (CBMAB-1154-CN)
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Mouse Anti-ATP1B1 Recombinant Antibody (E4) (CBMAB-0463-LY)
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Rabbit Anti-BAD (Phospho-Ser136) Recombinant Antibody (CAP219) (CBMAB-AP536LY)
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Mouse Anti-ARG1 Recombinant Antibody (CBYCL-103) (CBMAB-L0004-YC)
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Mouse Anti-BAD (Phospho-Ser136) Recombinant Antibody (CBYY-0138) (CBMAB-0139-YY)
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Rabbit Anti-ABL1 (Phosphorylated Y245) Recombinant Antibody (V2-505716) (PTM-CBMAB-0465LY)
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Mouse Anti-CD247 Recombinant Antibody (6B10.2) (CBMAB-C1583-YY)
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Mouse Anti-AAV-5 Recombinant Antibody (V2-503416) (CBMAB-V208-1402-FY)
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Mouse Anti-CD33 Recombinant Antibody (P67.6) (CBMAB-C10189-LY)
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Mouse Anti-CIITA Recombinant Antibody (CBLC160-LY) (CBMAB-C10987-LY)
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Mouse Anti-COL12A1 Recombinant Antibody (CBYY-C3117) (CBMAB-C4560-YY)
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Mouse Anti-DISP2 Monoclonal Antibody (F66A4B1) (CBMAB-1112CQ)
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Mouse Anti-BAX Recombinant Antibody (CBYY-0216) (CBMAB-0217-YY)
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Mouse Anti-BCL6 Recombinant Antibody (CBYY-0435) (CBMAB-0437-YY)
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Rat Anti-CCR2 Recombinant Antibody (475301) (CBMAB-C1338-LY)
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Mouse Anti-ABIN2 Recombinant Antibody (V2-179106) (CBMAB-A0349-YC)
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Rat Anti-ADGRE4 Recombinant Antibody (V2-160163) (CBMAB-F0011-CQ)
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Mouse Anti-ESR1 Recombinant Antibody (Y31) (CBMAB-1208-YC)
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Mouse Anti-BLK Recombinant Antibody (CBYY-0618) (CBMAB-0621-YY)
Membrane Antibody Arrays
Antibody arrays are valuable for the parallel analysis of multiple proteins in small sample volumes. The widely used application of antibody arrays has been for biomarker discovery and biological studies. Analogous to ELISA, antibody arrays are an antibody-pair-based assay. The first array approaches attempted to miniaturize biochemical and immunobiological assays performed in 96-well plates. But the small surface area in each well limits the number of antibody spots while 96-well plate-based antibody arrays have high-throughput capability. Nitrocellulose membranes and glass slides were subsequently utilized to develop arrays which could accommodate larger panels of antibodies. Nitrocellulose membrane-based arrays are flexible, easy to handle, and have increased protein binding capacity.
Fig. 1 Phospho-kinase antibody array.1
Creative Biolabs Membrane Antibody Arrays are used to measure many proteins simultaneously in one experiment, with significantly less time, effort and cost than ELISA or western blot assays. Moreover, membrane-based antibody arrays are very easy to set up and significantly cheaper compared to other multiplex immunoassays.
Membrane Antibody Arrays Workflow
- Spot capture antibodies on membrane
- Block membrane array with buffer
- Incubate protein samples (0.2-1 ml per sample)
- Wash off nonspecific proteins
- Incubate a cocktail of biotinylated detection antibodies
- Incubate streptavidin-conjugated fluorescence
- Visualize signals by fluorescence laser scanner
Please contact Creative Biolabs for membrane antibody arrays technology and related products.
Reference:
- Yu, Bingting, et al. "Drug discovery in liver disease using kinome profiling." International Journal of Molecular Sciences 22.5 (2021): 2623. Distributed under Open Access license CC BY 4.0, without modification.
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