CoV OC-43 Nucleoprotein

A reverse genetic system for a mouse neurovirulent strain of the human coronavirus OC43 (HCoV-OC43) was created using a BAC system similar to that used for TGEV and SARS-CoV. Like the TGEV and SARS-CoV systems described above, the HCoV-OC43 reverse genetic system relies on transcription from a CMV promoter to transcribe HCoV-OC43 genome RNAs from the transfected BAC containing a cDNA clone of the complete HCoV-OC43 genome. The recovered virus was neurovirulent for mice after intracranial injection, as was the parental virus.

Human coronavirus OC43