Antibody pair is two different antibodies developed against the same target that with different binding regions or epitopes. Differential epitope recognition renders simultaneous binding of both antibodies to the same target, to form sandwich ELISA format. Antibody pair can be used to detect and quantify interaction molecules in situ, ensure localization of sub-cellular events, reduce the risk of missing weak or transient interactions and improve detection specificity and sensitivity. Creative Biolabs has years of expertise in developing antibody pairs to provide a monoclonal antibody pair, a polyclonal antibody pair, or a combination for your specific requirement. Our high-quality products are suitable for used to quantify target analyte from complex samples across various fields including cancer, autoimmune, metabolic disease, endocrine, infectious disease, etc.
Principle of Antibody Pair
ELISA is designed to measure the amount of the target bound between a matched antibody pair. Firstly, a target-specific antibody (capture antibody) is coated to the bottom of the wells of a microplate, which is an overnight process. Then samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody. Thus, a sandwich is formed by the addition of the second antibody (detection antibody), and a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. Finally, the intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features of Our Products
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