CRISPR-Cas12

CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found within the genomes of prokaryotic organisms such as bacteria and archaea. Cas12a, previously known as Cpf1, due to its unique features and production of sticky ends when cleaving genetic sequences. To describe more of what makes it special, it should first be pointed out that Cas9 has two catalytic domains, places of active enzyme activity within the complex usually related to cleavage. These domains are called RuvC and HNH and are the most important parts of CRISPR, along with the guide RNA packaged with the complex, guiding them to the target sequence to bind to.

CRISPR-Cas12