DEF6

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Full Name

differentially expressed in FDCP 6

Function

Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling (By similarity).

Cellular Location

Cytoskeleton; Nucleus; Cell membrane; Cytoplasm; Perinuclear region; Filopodium. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate (PubMed:15023524). Binds to actin filaments (PubMed:15023524).

PTM

Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.

Anti-DEF6 antibodies

Mouse Anti-DEF6 Recombinant Antibody (1D1) (CBMAB-D0656-YC)

Datasheet

SDS

Target: DEF6

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 1D1

Application*: P, WB

Mouse Anti-DEF6 Recombinant Antibody (1E3) (CBMAB-D0657-YC)

Datasheet

SDS

Target: DEF6

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 1E3

Application*: IF, P, WB

Mouse Anti-DEF6 Recombinant Antibody (2G2) (CBMAB-D0659-YC)

Datasheet

SDS

Target: DEF6

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: 2G2

Application*: IF, P, WB

Mouse Anti-DEF6 Recombinant Antibody (3C4) (CBMAB-D0661-YC)

Datasheet

SDS

Target: DEF6

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 3C4

Application*: P, WB

Mouse Anti-DEF6 Recombinant Antibody (UMAB51) (CBMAB-D0664-YC)

Datasheet

SDS

Target: DEF6

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: UMAB51

Application*: P

Mouse Anti-DEF6 Recombinant Antibody (1F2) (CBMAB-D0666-YC)

Datasheet

SDS

Target: DEF6

Host: Mouse

Antibody Isotype: IgG2a, κ

Specificity: Human

Clone: 1F2

Application*: E, WB

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)