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Mouse Anti-DEF6 Recombinant Antibody (1D1) (CBMAB-D0656-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DEF6, Guanine Nucleotide Exchange Factor (DEF6). The antibody can be used for immunoassay techniques, such as IHC-P, WB.
See all DEF6 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D1
Antibody Isotype
IgG1
Application
IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
differentially expressed in FDCP 6
Introduction
DEF6 is a guanine nucleotide exchange factor (GEF) for RAC and CDC42 that is highly expressed in B and T cells.
Entrez Gene ID
50619
UniProt ID
Q9H4E7
Alternative Names
DEF6, Guanine Nucleotide Exchange Factor; SWAP-70-Like Adaptor Protein Of T Cells; IRF4-Binding Protein; DEF-6; IBP; Differentially Expressed In FDCP (Mouse Homolog) 6;
Function
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling (By similarity).
Cellular Location
Cytoskeleton; Nucleus; Cell membrane; Cytoplasm; Perinuclear region; Filopodium. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate (PubMed:15023524). Binds to actin filaments (PubMed:15023524).
PTM
Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.

Fournier, B., Tusseau, M., Villard, M., Malcus, C., Chopin, E., Martin, E., ... & Latour, S. (2021). DEF6 deficiency, a mendelian susceptibility to EBV infection, lymphoma, and autoimmunity. Journal of Allergy and Clinical Immunology, 147(2), 740-743.

Zhu, Z. P., Lin, L. R., Lv, T. D., Xu, C. R., Cai, T. Y., & Lin, J. (2020). High expression levels of DEF6 predicts a poor prognosis for patients with clear cell renal cell carcinoma. Oncology reports, 44(5), 2056-2066.

Cheng, H., & Sablitzky, F. (2019). The coiled coil domain of DEF6 facilitates formation of large vesicle-like, cytoplasmic aggregates that trap the P-body marker DCP1 and exhibit prion-like features. bioRxiv, 538082.

Serwas, N. K., Hoeger, B., Ardy, R. C., Stulz, S. V., Sui, Z., Memaran, N., ... & Boztug, K. (2019). Human DEF6 deficiency underlies an immunodeficiency syndrome with systemic autoimmunity and aberrant CTLA-4 homeostasis. Nature communications, 10(1), 1-15.

Cheng, H., Alsayegh, M., & Sablitzky, F. (2019). N-terminal 45 amino acids of DEF6 are necessary and sufficient to spontaneously colocalise with DCP1 in P-bodies. bioRxiv, 533109.

Alsayegh, M. (2018). DEF6 is associated with the translational initiation regulating protein synthesis of eIF4E, 4E-T and PABP and colocalises with eIF4E and PABP in the immunological synapse (Doctoral dissertation, University of Nottingham).

Cheng, H. (2018). Structure-function relationship of DEF6 (Doctoral dissertation, University of Nottingham).

Akdeniz, D. (2018). The roles of def6a and swap70b in zebrafish embryogenesis and haematopoiesis and DEF6 interactome analysis in Jurkat T cells (Doctoral dissertation, University of Nottingham).

Remon, K. (2017). DEF6 aggregation is linked to active translation and mRNA turnover in T cells (Doctoral dissertation, University of Nottingham).

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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