RBM4

RBM4 (RNA Binding Motif Protein 4) is a Protein Coding gene. Among its related pathways are Circadian rythm related genes and BMAL1-CLOCK,NPAS2 activates circadian gene expression. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding. An important paralog of this gene is RBM4B.

Full Name

RNA Binding Motif Protein 4

Function

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.

Biological Process

Biological Process cap-independent translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process circadian regulation of gene expressionIEA:Ensembl
Biological Process circadian regulation of translationISS:UniProtKB
Biological Process enteroendocrine cell differentiationIEA:Ensembl
Biological Process entrainment of circadian clock by photoperiodISS:UniProtKB
Biological Process insulin secretion involved in cellular response to glucose stimulusIEA:Ensembl
Biological Process IRES-dependent translational initiation of linear mRNAManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process miRNA-mediated gene silencing by inhibition of translationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of translationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of translation in response to stressManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process pancreas developmentIEA:Ensembl
Biological Process positive regulation of muscle cell differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of translationIEA:Ensembl
Biological Process regulation of alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of insulin receptor signaling pathwayIEA:Ensembl
Biological Process regulation of nucleocytoplasmic transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process response to arsenic-containing substanceManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process RNA processingManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus
Nucleus, nucleolus
Nucleus speckle
Cytoplasm
Cytoplasmic granule
Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress-induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation.

PTM

Phosphorylation on Ser-309 is induced upon cell muscle differentiation (By similarity).
Phosphorylated. Phosphorylated in vitro on Ser-309 by SRPK1. Phosphorylation on Ser-309 is induced upon cell stress signaling, which alters its subcellular localization and may modulate its activity on IRES-mediated mRNA translation.