Mouse Anti-RBM4 Recombinant Antibody (CF365) (CBMAB-FT144LY)

RNA Binding Motif Protein 4

RBM4 (RNA Binding Motif Protein 4) is a Protein Coding gene. Among its related pathways are Circadian rythm related genes and BMAL1-CLOCK,NPAS2 activates circadian gene expression. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding. An important paralog of this gene is RBM4B.

RBM4A

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.

Biological Process cap-independent translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process circadian regulation of gene expressionIEA:Ensembl
Biological Process circadian regulation of translationISS:UniProtKB
Biological Process enteroendocrine cell differentiationIEA:Ensembl
Biological Process entrainment of circadian clock by photoperiodISS:UniProtKB
Biological Process insulin secretion involved in cellular response to glucose stimulusIEA:Ensembl
Biological Process IRES-dependent translational initiation of linear mRNAManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process miRNA-mediated gene silencing by inhibition of translationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of translationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of translation in response to stressManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process pancreas developmentIEA:Ensembl
Biological Process positive regulation of muscle cell differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of translationIEA:Ensembl
Biological Process regulation of alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of insulin receptor signaling pathwayIEA:Ensembl
Biological Process regulation of nucleocytoplasmic transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process response to arsenic-containing substanceManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process RNA processingManual Assertion Based On ExperimentTAS:ProtInc

Nucleus
Nucleus, nucleolus
Nucleus speckle
Cytoplasm
Cytoplasmic granule
Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress-induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation.

Phosphorylation on Ser-309 is induced upon cell muscle differentiation (By similarity).
Phosphorylated. Phosphorylated in vitro on Ser-309 by SRPK1. Phosphorylation on Ser-309 is induced upon cell stress signaling, which alters its subcellular localization and may modulate its activity on IRES-mediated mRNA translation.