AGO1

AGO1 is a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translat

Full Name

Argonaute 1, RISC Catalytic Component

Function

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

Biological Process

Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
Negative regulation of angiogenesis
Negative regulation of gene expression
Nuclear-transcribed mRNA catabolic process
Positive regulation of gene expression
Positive regulation of transcription by RNA polymerase II
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of transcription initiation from RNA polymerase II promoter
RNA phosphodiester bond hydrolysis, endonucleolytic
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway

Cellular Location

P-body

PTM

Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO1 when it is engaged with a TDMD target.