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Rabbit Anti-AGO1 Recombinant Antibody (V2-180164) (CBMAB-A1585-YC)

Provided herein is a Rabbit monoclonal antibody against Human Argonaute 1, RISC Catalytic Component. The antibody can be used for immunoassay techniques, such as WB, IP, IHC-P.
See all AGO1 antibodies
Published Data
Rabbit Anti-AGO1 Recombinant Antibody (D84G10) (CBMAB-A1585-YC) in WB
Change in confluency over time of HeLa parental or AGO2 k.o. cells transfected with four of the HPs at 25 nM. ...View More
Murmann, A. E., Patel, M., Jeong, S. Y., Bartom, E. T., Jennifer Morton, A., & Peter, M. E. (2022). The length of uninterrupted CAG repeats in stem regions of repeat disease associated hairpins determines the amount of short CAG oligonucleotides that are toxic to cells through RNA interference. Cell Death & Disease, 13(12), 1078.
Rabbit Anti-AGO1 Recombinant Antibody (D84G10) (CBMAB-A1585-YC) in Immunoblot
Immunoblot analysis of DROSHA, DICER, AGO1, and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. ...View More
Schaefer, M., Nabih, A., Spies, D., Hermes, V., Bodak, M., Wischnewski, H., ... & Ciaudo, C. (2022). Global and precise identification of functional miRNA targets in mESCs by integrative analysis. EMBO reports, 23(9), e54762.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
V2-180164
Antibody Isotype
IgG
Application
WB, IP, IHC-P

Basic Information

Immunogen
Synthetic peptide corresponding to the sequence around Ile392 of human Argonaute 1 protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50
IHC-P1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Argonaute 1, RISC Catalytic Component
Introduction
AGO1 is a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translat
Entrez Gene ID
Human26523
Mouse236511
Rat313594
Monkey712398
UniProt ID
HumanQ9UL18
MouseQ8CJG1
RatD4AC38
MonkeyH9FSW2
Alternative Names
Argonaute 1, RISC Catalytic Component; Argonaute RISC Catalytic Component 1; Eukaryotic Translation Initiation Factor 2C, 1; Putative RNA-Binding Protein Q99; Argonaute1; EIF-2C 1; EIF2C 1; EIF2C1; HAgo1;
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
Biological Process
Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
Negative regulation of angiogenesis
Negative regulation of gene expression
Nuclear-transcribed mRNA catabolic process
Positive regulation of gene expression
Positive regulation of transcription by RNA polymerase II
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of transcription initiation from RNA polymerase II promoter
RNA phosphodiester bond hydrolysis, endonucleolytic
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway
Cellular Location
P-body
PTM
Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO1 when it is engaged with a TDMD target.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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