Human Recombinant AGO1 protein, His Tag (V2LY-0526-LY1957)

Name: Human Recombinant AGO1 protein, His Tag
SKU: V2LY-0526-LY1957
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant AGO1 protein, His Tag consist of Amino Acid: 1-857 and predicts a molecular mass of 99.5 kDa.

Molecule Mass

99.5 kDa

Sequence

Amino Acid: 1-857

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Argonaute 1, RISC Catalytic Component

Function

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

Biological Process

Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
Negative regulation of angiogenesis
Negative regulation of gene expression
Nuclear-transcribed mRNA catabolic process
Positive regulation of gene expression
Positive regulation of transcription by RNA polymerase II
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of transcription initiation from RNA polymerase II promoter
RNA phosphodiester bond hydrolysis, endonucleolytic
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway

Cellular Location

P-body

PTM

Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO1 when it is engaged with a TDMD target.

Nowak, I., & Sarshad, A. A. (2021). Argonaute Proteins Take Center Stage in Cancers. Cancers, 13(4), 788.

Fawzy, M. S., Toraih, E. A., Alelwani, W., Kattan, S. W., Alnajeebi, A. M., & Hassan, R. (2020). The prognostic value of microRNA-biogenesis genes Argonaute 1 and 2 variants in breast cancer patients. American Journal of Translational Research, 12(5), 1994.

Shen, Q., Wang, Y. E., Truong, M., Mahadevan, K., Wu, J. Z., Smith, H. W., ... & Palazzo, A. F. (2020). RanBP2/Nup358 enhances miRNA activity by sumoylating and stabilizing Argonaute 1. BioRxiv, 555896.

Wu, G., Fan, F., Hu, P., & Wang, C. (2020). AGO1 enhances the proliferation and invasion of cholangiocarcinoma via the EMT-associated TGF-β signaling pathway. American Journal of Translational Research, 12(6), 2890.

Li, J., Bao, H., Zhang, K., Yang, X., Liu, X., Li, P., ... & Chen, W. (2020). MiR-542-3p drives renal fibrosis by targeting AGO1 in vivo and in vitro. Life Sciences, 255, 117845.

Kenesi, E., Carbonell, A., Lozsa, R., Vértessy, B., & Lakatos, L. (2017). A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC. Nucleic acids research, 45(13), 7736-7750.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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