Rat Anti-AGO1 Recombinant Antibody (V2-43351) (CBMAB-E0578-FY)

Name: Rat Anti-AGO1 Recombinant Antibody (V2-43351)
SKU: CBMAB-E0578-FY
Rating: 5 (1 reviews)

See all AGO1 antibodies

Online Inquiry

Datasheet

Summary

Host Animal

Rat

Specificity

Human

Clone

V2-43351

Antibody Isotype

IgG1, κ

Application

Basic Information

Immunogen

GST-tagged recombinant human Ago1.

Host Species

Rat

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1 M Tris-Glycine, pH 7.4

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Argonaute 1, RISC Catalytic Component

Introduction

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Entrez Gene ID

26523

UniProt ID

Q9UL18

Alternative Names

Argonaute 1, RISC Catalytic Component; Argonaute RISC Catalytic Component 1; Eukaryotic Translation Initiation Factor 2C, 1; Putative RNA-Binding Protein Q99; Argonaute1; EIF-2C 1; EIF2C 1; EIF2C1; HAgo1

Function

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

Biological Process

Intracellular receptor signaling pathway
miRNA loading onto RISC involved in gene silencing by miRNA
miRNA mediated inhibition of translation
Negative regulation of angiogenesis
Negative regulation of gene expression
Nuclear-transcribed mRNA catabolic process
Positive regulation of gene expression
Positive regulation of transcription by RNA polymerase II
Post-transcriptional gene silencing by RNA
Pre-miRNA processing
Production of miRNAs involved in gene silencing by miRNA
Regulation of gene silencing by miRNA
Regulation of megakaryocyte differentiation
Regulation of transcription initiation from RNA polymerase II promoter
RNA phosphodiester bond hydrolysis, endonucleolytic
RNA secondary structure unwinding
Wnt signaling pathway, calcium modulating pathway

Cellular Location

P-body

PTM

Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO1 when it is engaged with a TDMD target.

More Infomation

References

Nowak, I., & Sarshad, A. A. (2021). Argonaute Proteins Take Center Stage in Cancers. Cancers, 13(4), 788.

Fawzy, M. S., Toraih, E. A., Alelwani, W., Kattan, S. W., Alnajeebi, A. M., & Hassan, R. (2020). The prognostic value of microRNA-biogenesis genes Argonaute 1 and 2 variants in breast cancer patients. American Journal of Translational Research, 12(5), 1994.

Shen, Q., Wang, Y. E., Truong, M., Mahadevan, K., Wu, J. Z., Smith, H. W., ... & Palazzo, A. F. (2020). RanBP2/Nup358 enhances miRNA activity by sumoylating and stabilizing Argonaute 1. BioRxiv, 555896.

Wu, G., Fan, F., Hu, P., & Wang, C. (2020). AGO1 enhances the proliferation and invasion of cholangiocarcinoma via the EMT-associated TGF-β signaling pathway. American Journal of Translational Research, 12(6), 2890.

Li, J., Bao, H., Zhang, K., Yang, X., Liu, X., Li, P., ... & Chen, W. (2020). MiR-542-3p drives renal fibrosis by targeting AGO1 in vivo and in vitro. Life Sciences, 255, 117845.

Kenesi, E., Carbonell, A., Lozsa, R., Vértessy, B., & Lakatos, L. (2017). A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC. Nucleic acids research, 45(13), 7736-7750.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Rat Anti-AGO1 Recombinant Antibody (V2-43351)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Rabbit Anti-AGO1 Recombinant Antibody (V2-180164)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: