FNDC5

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants.

Full Name

Fibronectin Type III Domain Containing 5

Function

Irisin:
Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.

Biological Process

Positive regulation of brown fat cell differentiation Source: UniProtKB
Response to muscle activity Source: UniProtKB

Cellular Location

Peroxisome membrane; Cell membrane. Imported in peroxisomes through the PEX5 receptor pathway.
Irisin: Secreted. Detected in the blood of individuals subjected to endurance exercise.

Topology

Extracellular: 32-152
Helical: 153-173
Cytoplasmic: 174-212

PTM

The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.

Anti-FNDC5 antibodies

Rabbit Anti-FNDC5 Recombinant Antibody (CBXF-1096) (CBMAB-F0932-CQ)

Datasheet

SDS

Target: FNDC5

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat

Clone: CBXF-1096

Application*: WB

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)