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CD28

CD28 (CD28 Molecule) is a Protein Coding gene. Diseases associated with CD28 include Mycosis Fungoides and Sezary's Disease. Among its related pathways are Th2 Differentiation Pathway and RET signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and SH3/SH2 adaptor activity. An important paralog of this gene is CTLA4.
Full Name
CD28 Molecule
Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933).
Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Biological Process
Apoptotic signaling pathway Source: Ensembl
Cell surface receptor signaling pathway Source: UniProtKB
Humoral immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of alpha-beta T cell proliferation Source: Ensembl
Positive regulation of cytokine production Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of inflammatory response to antigenic stimulus Source: Ensembl
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of isotype switching to IgG isotypes Source: Ensembl
Positive regulation of mitotic nuclear division Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of translation Source: UniProtKB
Positive regulation of viral genome replication Source: UniProtKB
Regulation of defense response to virus by virus Source: Reactome
Regulation of regulatory T cell differentiation Source: Ensembl
Regulatory T cell differentiation Source: BHF-UCL
T cell activation Source: BHF-UCL
T cell costimulation Source: GO_Central
T cell receptor signaling pathway Source: GO_Central
Cellular Location
Membrane
Isoform 3: Cell surface
Topology
Extracellular: 19-152
Helical: 153-179
Cytoplasmic: 180-220
PTM
CD40LG induces tyrosine phosphorylation of isoform 3.

Anti-CD28 antibodies

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Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBXC-1464
Application*: F, IH
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBXC-1380
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Mouse
Clone: E18
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: C28/1636
Application*: F, IF
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse, Pig, Sheep
Clone: CBXC-2714
Application*: F, IF
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Dog
Clone: CBFYC-1211
Application*: F, FN
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYC-1210
Application*: F, WB
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYC-1209
Application*: F, IF
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Chicken
Clone: CBFYC-1208
Application*: F
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBFYC-1207
Application*: IH, F
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBFYC-1205
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBFYC-0427
Application*: F, C
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYC-0202
Application*: F, IC, IF
Target: CD28
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBFYC-0062
Application*: E, F
Target: CD28
Host: Hamster
Antibody Isotype: IgG, λ
Specificity: Mouse
Clone: CBXC-1562
Application*: F, IP
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: ANC28.1/5D10
Application*: E, F, FN
Target: CD28
Host: Human
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXC-0895
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXC-1150
Application*: F
Target: CD28
Host: Rat
Antibody Isotype: IgG2b
Specificity: Human
Clone: YTH913.12
Application*: F, IH, C
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CD28.2
Application*: WB, F, IH, IF, C
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CB28
Application*: F, IF, C
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: C28/77
Application*: F, IF, IP, P, C
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: C28/75
Application*: F, IF, P
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 15E8
Application*: F
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: MAB0818
Application*: B, S
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: AP-MAB0711
Application*: F
Target: CD28
Host: Rat
Antibody Isotype: IgG2b
Specificity: Human
Clone: YTH 913.12
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 5D10
Application*: E, FC
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: A044
Application*: FC, IHC, IP
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: A043
Application*: FC, IHC, IP
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Cattle, Human, Pig, Sheep, Mouse
Clone: 204-12
Application*: FA, FC, IF
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: B-23
Application*: FC
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CAP389
Application*: FC
Target: CD28
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: D2Z4E
Application*: WB, IP
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 3E12
Application*: WB, E
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: AP-MAB0818
Application*: B/N
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Chicken
Clone: AV7
Application*: F
Target: CD28
Host: Mouse
Specificity: Human
Clone: 4B5
Application*: E, WB
Target: CD28
Host: Rabbit
Antibody Isotype: IgG2b
Specificity: Human
Clone: 3H1179
Application*: FC
Target: CD28
Host: Mouse
Specificity: Human
Clone: 1D7
Application*: E, WB
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 14L187
Application*: FC, WB
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: B-T3
Application*: FC
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBYY-C1310
Application*: S
Target: CD28
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBYY-C1309
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Chicken
Clone: CBYY-C1308
Application*: F, IH, IP
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBYY-C0605
Application*: E, F
Target: CD28
Host: Rat
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBYY-C0519
Application*: F, IH, IP
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 9.3
Application*: A
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Chicken
Clone: CBYY-C0478
Application*: F, IP, WB
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: 5K12
Application*: F, IP
Target: CD28
Host: Rat
Antibody Isotype: IgG2c
Specificity: Mouse
Clone: 61109
Application*: WB
Target: CD28
Host: Rat
Antibody Isotype: IgG2b
Specificity: Human
Clone: 6A266
Application*: F, IH
Target: CD28
Host: Rat
Antibody Isotype: IgG1
Specificity: Mouse
Clone: 794716
Application*: F, AG, MC
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 7H132
Application*: E, F
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: MAB0711
Application*: C, F
Target: CD28
Host: Rat
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBYY-C0101
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Dog
Clone: CBYY-C0353
Application*: F
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBYY-C0422
Application*: F, IP
Target: CD28
Host: Hamster
Antibody Isotype: IgG2
Specificity: Mouse
Clone: 37.51
Application*: A, B
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Rat
Clone: JJ319
Application*: A
Target: CD28
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: 007
Application*: E, F
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 10F3
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human, Mouse
Clone: 204.12
Application*: F, IH, C, MC
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: 37.51.1
Application*: F
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 37407
Application*: WB, F, AG, MC
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: 37-51
Application*: C, FN, C, P, F
Target: CD28
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: 37-51-1
Application*: C, F
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Chicken
Clone: 39482
Application*: F, IH, IP, C
Target: CD28
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: 04
Application*: ED
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 12B3
Application*: C, WB, A
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 13H106
Application*: F, IF
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 13H107
Application*: E
Target: CD28
Host: Rat
Antibody Isotype: IgG2c
Specificity: Mouse
Clone: CBYY-C0494
Application*: WB
Target: CD28
Host: Rat
Antibody Isotype: IgG
Specificity: Mouse
Clone: 15H16
Application*: WB
Target: CD28
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: 1D5
Application*: E, WB
Target: CD28
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Chicken
Clone: 5K85
Application*: F, IP, WB
Target: CD28
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Chicken
Clone: 4j11
Application*: F, IH, IP
Target: CD28
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXC-0204
Application*: F
Target: CD28
Host: Rat
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBXC-0203
Application*: F, IH
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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