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Hamster Anti-CD28 Recombinant Antibody (CBFYC-1205) (CBMAB-C1263-FY)

This product is hamster antibody that recognizes CD28. The antibody CBFYC-1205 can be used for immunoassay techniques such as: FC.
See all CD28 antibodies

Summary

Host Animal
Hamster
Specificity
Mouse
Clone
CBFYC-1205
Antibody Isotype
IgG
Application
FC

Basic Information

Immunogen
CD28 costimulatory receptor
Specificity
Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD28 Molecule
Introduction
CD28 (CD28 Molecule) is a Protein Coding gene. Diseases associated with CD28 include Mycosis Fungoides and Sezary's Disease. Among its related pathways are Th2 Differentiation Pathway and RET signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and SH3/SH2 adaptor activity. An important paralog of this gene is CTLA4.
Entrez Gene ID
UniProt ID
Alternative Names
T-cell-specific surface glycoprotein CD28
Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933).
Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Biological Process
Apoptotic signaling pathway Source: Ensembl
Cell surface receptor signaling pathway Source: UniProtKB
Humoral immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of alpha-beta T cell proliferation Source: Ensembl
Positive regulation of cytokine production Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of inflammatory response to antigenic stimulus Source: Ensembl
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of isotype switching to IgG isotypes Source: Ensembl
Positive regulation of mitotic nuclear division Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of translation Source: UniProtKB
Positive regulation of viral genome replication Source: UniProtKB
Regulation of defense response to virus by virus Source: Reactome
Regulation of regulatory T cell differentiation Source: Ensembl
Regulatory T cell differentiation Source: BHF-UCL
T cell activation Source: BHF-UCL
T cell costimulation Source: GO_Central
T cell receptor signaling pathway Source: GO_Central
Cellular Location
Membrane
Isoform 3: Cell surface
Topology
Extracellular: 19-152
Helical: 153-179
Cytoplasmic: 180-220
PTM
CD40LG induces tyrosine phosphorylation of isoform 3.

Matundan, H. H., Jaggi, U., Yu, J., Akbari, O., & Ghiasi, H. (2021). Absence of CD28-CTLA4-PD-L1 Costimulatory Molecules Reduces Herpes Simplex Virus 1 Reactivation. Mbio, 12(4), e01176-21.

Nagai, S., & Azuma, M. (2019). The CD28–B7 family of co-signaling molecules. Co-signal Molecules in T Cell Activation, 25-51.

Huff, W. X., Kwon, J. H., Henriquez, M., Fetcko, K., & Dey, M. (2019). The evolving role of CD8+ CD28− immunosenescent T cells in cancer immunology. International journal of molecular sciences, 20(11), 2810.

Hosoe, Y., Numoto, N., Inaba, S., Ogawa, S., Morii, H., Abe, R., ... & Oda, M. (2019). Structural and functional properties of Grb2 SH2 dimer in CD28 binding. Biophysics and physicobiology, 16, 80-88.

Siokis, A., Robert, P. A., Demetriou, P., Dustin, M. L., & Meyer-Hermann, M. (2018). F-actin-driven CD28-CD80 localization in the immune synapse. Cell reports, 24(5), 1151-1162.

Huang, Y., Wang, Z., Zheng, Q., Tang, J., Cai, J., Lu, Y., & Jian, J. (2018). Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 72, 95-103.

Xia, F., Qian, C. R., Xun, Z., Hamon, Y., Sartre, A. M., Formisano, A., ... & He, H. T. (2018). TCR and CD28 concomitant stimulation elicits a distinctive calcium response in naive T cells. Frontiers in immunology, 9, 2864.

Porciello, N., Grazioli, P., Campese, A. F., Kunkl, M., Caristi, S., Mastrogiovanni, M., ... & Tuosto, L. (2018). A non-conserved amino acid variant regulates differential signalling between human and mouse CD28. Nature communications, 9(1), 1-16.

Yang, W., Pan, W., Chen, S., Trendel, N., Jiang, S., Xiao, F., ... & Xu, C. (2017). Dynamic regulation of CD28 conformation and signaling by charged lipids and ions. Nature structural & molecular biology, 24(12), 1081-1092.

Zumerle, S., Molon, B., & Viola, A. (2017). Membrane rafts in T cell activation: a spotlight on CD28 costimulation. Frontiers in immunology, 8, 1467.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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