Mouse Anti-CD28 Recombinant Antibody (CD28.2) (CBMAB-C0532-CQ)

Name: Mouse Anti-CD28 Recombinant Antibody (CD28.2)
SKU: CBMAB-C0532-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CD28.2

Application

in vivo, FC, IHC, IP

Immunogen

Human CD28 cDNA

Host Species

Mouse

Specificity

Human, Monkey

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CD28 Molecule

Introduction

CD28 (CD28 Molecule) is a Protein Coding gene. Diseases associated with CD28 include Mycosis Fungoides and Sezary's Disease. Among its related pathways are Th2 Differentiation Pathway and RET signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and SH3/SH2 adaptor activity. An important paralog of this gene is CTLA4.

Entrez Gene ID

940

UniProt ID

P10747

Alternative Names

CD28 Molecule; CD28 Antigen; Tp44; T-Cell-Specific Surface Glycoprotein CD28; T-Cell-Specific Surface Glycoprotein; CD28 Antigen (Tp44);

Function

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933).
Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Biological Process

Apoptotic signaling pathway Source: Ensembl
Cell surface receptor signaling pathway Source: UniProtKB
Humoral immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of alpha-beta T cell proliferation Source: Ensembl
Positive regulation of cytokine production Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of inflammatory response to antigenic stimulus Source: Ensembl
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of isotype switching to IgG isotypes Source: Ensembl
Positive regulation of mitotic nuclear division Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of translation Source: UniProtKB
Positive regulation of viral genome replication Source: UniProtKB
Regulation of defense response to virus by virus Source: Reactome
Regulation of regulatory T cell differentiation Source: Ensembl
Regulatory T cell differentiation Source: BHF-UCL
T cell activation Source: BHF-UCL
T cell costimulation Source: GO_Central
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Membrane
Isoform 3: Cell surface

Topology

Extracellular: 19-152
Helical: 153-179
Cytoplasmic: 180-220

PTM

CD40LG induces tyrosine phosphorylation of isoform 3.

Matundan, H. H., Jaggi, U., Yu, J., Akbari, O., & Ghiasi, H. (2021). Absence of CD28-CTLA4-PD-L1 Costimulatory Molecules Reduces Herpes Simplex Virus 1 Reactivation. Mbio, 12(4), e01176-21.

Nagai, S., & Azuma, M. (2019). The CD28–B7 family of co-signaling molecules. Co-signal Molecules in T Cell Activation, 25-51.

Huff, W. X., Kwon, J. H., Henriquez, M., Fetcko, K., & Dey, M. (2019). The evolving role of CD8+ CD28− immunosenescent T cells in cancer immunology. International journal of molecular sciences, 20(11), 2810.

Hosoe, Y., Numoto, N., Inaba, S., Ogawa, S., Morii, H., Abe, R., ... & Oda, M. (2019). Structural and functional properties of Grb2 SH2 dimer in CD28 binding. Biophysics and physicobiology, 16, 80-88.

Siokis, A., Robert, P. A., Demetriou, P., Dustin, M. L., & Meyer-Hermann, M. (2018). F-actin-driven CD28-CD80 localization in the immune synapse. Cell reports, 24(5), 1151-1162.

Huang, Y., Wang, Z., Zheng, Q., Tang, J., Cai, J., Lu, Y., & Jian, J. (2018). Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 72, 95-103.

Xia, F., Qian, C. R., Xun, Z., Hamon, Y., Sartre, A. M., Formisano, A., ... & He, H. T. (2018). TCR and CD28 concomitant stimulation elicits a distinctive calcium response in naive T cells. Frontiers in immunology, 9, 2864.

Porciello, N., Grazioli, P., Campese, A. F., Kunkl, M., Caristi, S., Mastrogiovanni, M., ... & Tuosto, L. (2018). A non-conserved amino acid variant regulates differential signalling between human and mouse CD28. Nature communications, 9(1), 1-16.

Yang, W., Pan, W., Chen, S., Trendel, N., Jiang, S., Xiao, F., ... & Xu, C. (2017). Dynamic regulation of CD28 conformation and signaling by charged lipids and ions. Nature structural & molecular biology, 24(12), 1081-1092.

Zumerle, S., Molon, B., & Viola, A. (2017). Membrane rafts in T cell activation: a spotlight on CD28 costimulation. Frontiers in immunology, 8, 1467.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CD28 Matched Antibody Pair (216) (CAT#: APMAB-216LY)

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Isotype control

For research use only. Not intended for any clinical use.

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