COL25A1

COL25A1 is a brain-specific membrane-bound collagen. Proteolytic processing releases CLAC, a soluble form of COL25A1 containing the extracellular collagen domains that associates with senile plaques in Alzheimer disease (AD; MIM 104300) brains (Osada et al., 2005 [PubMed 15615705]).[supplied by OMIM

Full Name

collagen, type XXV, alpha 1

Function

Inhibits fibrillization of amyloid-beta peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin.

Biological Process

Collagen fibril organization Source: Reactome

Cellular Location

Membrane. After proteolytic cleavage, CLAC is secreted.

Involvement in disease

Fibrosis of extraocular muscles, congenital, 5 (CFEOM5):
An ocular motility disorder characterized by congenital dysinnervation of various cranial nerves to ocular muscles. Clinical features are ophthalmoplegia, anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head.

Topology

Cytoplasmic: 1-33
Helical: 34-54
Extracellular: 55-654

PTM

Undergoes proteolytic cleavage by furin protease to yield the soluble collagen-like Alzheimer amyloid plaque component.
Glycosylated.
Hydroxylated on 11% of proline residues and 49% of lysine residues.

Anti-COL25A1 antibodies

Mouse Anti-COL25A1 (AA 57-122) Recombinant Antibody (CBFYC-2050) (CBMAB-C2116-FY)

Datasheet

SDS

Target: COL25A1

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: CBFYC-2050

Application*: E, WB

Mouse Anti-COL25A1 (AA 189-654) Recombinant Antibody (CBFYC-2049) (CBMAB-C2115-FY)

Datasheet

SDS

Target: COL25A1

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: CBFYC-2049

Application*: E, IH

Mouse Anti-COL25A1 Recombinant Antibody (CBYY-C0321) (CBMAB-C1080-YY)

Datasheet

SDS

Target: COL25A1

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: CBYY-C0321

Application*: IH

Mouse Anti-COL25A1 Recombinant Antibody (CBLNC-143) (CBMAB-1411-CN)

Datasheet

SDS

Target: COL25A1

Host: Mouse

Antibody Isotype: IgG

Specificity: Human

Clone: CBLNC-143

Application*: IH

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)