AKT/PKB Pathway Phosphorylation Antibody Array
AKT/PKB is a serine/threonine kinase, which controls vital cellular functions such as cell survival/apoptosis, cell cycle progression and glucose metabolism. The AKT/PKB signaling pathway is a signal transduction pathway where key proteins are phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT). AKT/PKB carries out these diverse tasks through phosphorylation of several cellular substrates. The substrates of AKT/PKB promote the inhibition of apoptosis after being phosphorylated by AKT.
Fig.1 PI3K/Akt signaling and cancer.1
AKT protein kinase plays an important role in cell, tissue, and organismal physiology. The ever-expanding list of AKT substrates and their overlap creates a dynamic environment where the integration of distinct signals at the level of multiple downstream effectors is required for a cell to mount an appropriate response. Creative Biolabs developed AKT/PKB signaling pathway phosphorylation screening service with antibody array, which consists of 216 high-specific antibodies and more than 100 phosphorylation sites involved in AKT/PKB signaling pathways can be tested.
Related Signaling Pathway
- AKT/PKB signaling pathway
Antibody Array Targets
| Targets |
| 14-3-3 theta/tau (S232) | eNOS (T495) | IRS-1 (S639) | p70S6K (S424) |
| 14-3-3 zeta (S58) | FAK (S910) | IRS-1 (S794) | p70S6K (T229) |
| 14-3-3 zeta/delta (T232) | FAK (Y397) | JAK1 (Y1022) | p70S6K (T389) |
| PFKFB2 (S483) | FAK (Y407) | LYN (Y507) | p70S6K (T421) |
| AKT (S473) | FAK (Y576) | MDM2 (S166) | p70S6K-beta (S423) |
| AKT (T308) | FAK (Y861) | mTOR (S2448) | Paxillin (Y118) |
| AKT (Y326) | FAK (Y925) | mTOR (S2481) | Paxillin (Y31) |
| AKT1 (S124) | FKHR (S256) | mTOR (T2446) | PDK1 (S241) |
| AKT1 (S246) | FKHR (S319) | p21Cip1 (T145) | PIK3R1 (Y607) |
| AKT1 (T450) | FOXO1/3/4 pan (T24/T32) | p27Kip1 (S10) | PIK3R1/PIK3R3 (Y467/Y199) |
| AKT1 (T72) | FOXO1A (S329) | p27Kip1 (T187) | PIP5K (S307) |
| AKT1 (Y474) | FOXO1A/3A (S322/S325) | p53 (S15) | PP2A-a (Y307) |
| AKT1S1 (T246) | Gab1 (Y627) | p53 (S20) | PTEN (S370) |
| AKT2 (S474) | Gab1 (Y659) | p53 (S315) | PTEN (S380) |
| BAD (S112) | Gab2 (Y643) | p53 (S33) | PTEN (S380/T382/T383) |
| BAD (S134) | GABA-RB (S434) | p53 (S366) | RapGEF1 (Y504) |
| BAD (S136) | GSK3a-b (Y216/Y279) | p53 (S37) | rpS6 (S235) |
| BAD (S155) | GSK3α (S21) | p53 (S378) | SYK (Y323) |
| BAD (S91/S128) | GSK3β (S9) | p53 (S392) | SYK (Y348) |
| BCL-2 (S70) | IKKα (T23) | p53 (S46) | SYK (Y525) |
| BCL-2 (S87) | IKKa/b (S180/S181) | p53 (S6) | SYN1 (S62) |
| BCL-2 (T56) | IRS-1 (S1101) | p53 (S9) | TSC2 (S939) |
| BCL-2 (T69) | IRS-1 (S307) | p53 (T18) | TSC2 (T1462) |
| BIM (S69/65) | IRS-1 (S312) | p53 (T81) | WEE1 (S642) |
| CCND1 (T286) | IRS-1 (S323) | p70S6K (S371) | XIAP (S87) |
| eNOS (S1177) | IRS-1 (S612) | p70S6K (S411) | |
| eNOS (S615) | IRS-1 (S636) | p70S6K (S418) |
Protocol Outline
- Extracted protein and biotinylated
- Blocked antibody array
- Incubate with treated protein and control
- Incubate with streptavidin-conjugated fluorophore
- Scan with a microarray laser scanner
- Perform densitometry and analysis
Our Analysis Service
- Suitable for protein, cell and tissue samples from human, mouse and rat
- 6×107 cells or 150 µg protein
- Set replicate spots: treated sample and control
- Results are delivered in 4-5 weeks
For more detailed information about our antibody arrays, please feel free to contact us.
Reference
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He, Yan, et al. "Targeting PI3K/Akt signal transduction for cancer therapy." Signal transduction and targeted therapy 6.1 (2021): 425.
Distributed under Open Access license CC BY 4.0, without modification.