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Apoptosis Pathway Phosphorylation Antibody Array

Apoptosis is a kind of programmed cell death (PCD) that plays a fundamental role in genomic DNA fragmentation, cell membrane blebbing, chromatin condensation and exposure of specific phagocytosis signaling molecules on the cell surface. There are two major signaling pathways that lead to apoptosis: the intrinsic pathway and the extrinsic pathway. In the intrinsic pathway, the cell kills itself because it senses cell stress. The extrinsic pathway is initiated when death receptors at the cell surface encounter specific cognate “death ligands”, which can activate caspases within seconds of ligand binding.

Apoptosis signaling pathwaysFig.1 Apoptosis signaling pathways[1].

Further studies in apoptosis signal pathway will undoubtedly reveal novel information that advance our understanding of its regulatory mechanisms and treament of diseases, such as neurodegenerative diseases, cancer, or autoimmunity. Creative Biolabs offers apoptosis signal pathway phosphorylation screening service with antibody array, which consists of 214 high-specific antibodies and more than 110 phosphorylation sites involved in apoptosis signal pathways can be tested.

Related Signaling Pathway

Signal Pathways[2]
  • TNFα-TNFR1 signaling pathway
  • TRAIL-DR signaling pathway
  • NF-KB signaling pathway
  • Fas-FasL signaling pathway

Antibody Array Targets

Targets
14-3-3 theta/tau (S232) CASP6 (S257) HSP27 (S78) NFkB-p65 (S529)
14-3-3 zeta (S58) CASP8 (S347) HSP27 (S82) NFkB-p65 (S536)
14-3-3 zeta/delta (T232) CASP9 (T125) HSP90B (S254) NFkB-p65 (T254)
AKT (S473) CASP9 (S144) NFKBIA (S32/S36) p53 (S37)
AKT (T308) CASP9 (S196) NFKBIA (Y42) p53 (S392)
AKT1 (T72) CASP9 (Y153) NFKBIB (T19) p53 (S6)
AKT1 (Y474) CASP3 (S150) NFKBIE (S22) p70S6K (S411)
AKT2 (S474) CDK1 (Y15) CHUK (T23) p70S6K (S424)
A-RAF (Y301/Y302) CDK1 (T14) IKBKB (Y188) p70S6K (T229)
ASK1 (S83) Chk1 (S280) IKBKB (Y199) p70S6K (T389)
ASK1 (S966) Chk1 (S286) IKBKG (S85) p70S6k (T421)
ATRIP (S68/S72) Chk1 (S317) IKKa/b (S180/S181) p70S6K (S371)
BAD (S112) Chk1 (S345) IKBKG (S31) p70S6K (S418)
BAD (S134) Chk2 (S516) JNK1/2/3 (T183/Y185) RPS6KB1 (S423)
BAD (S136) Chk2 (T383) Lamin A/B (S392) P90RSK (S380)
BAD (S155) Chk2 (T387) Lamin A/C (S22) P90RSK (T359/S363)
BAD (S91/S128) Chk2 (T68) MAP2K7 (S271) P90RSK (T573)
BCL2 (S70) c-Jun (S243) MAP2K7 (T275) PIK3R1/PIK3R3 (Y467/Y199)
BCL2 (S87) c-Jun (T91) NFkB-p100 (S872) PKA CAT (T197)
BCL2 (T56) DAXX (S668) NFkB-p100/p52 (S865) PKC pan activation site
BCL2 (T69) Erk1/2 (T202) NFkB-p100/p52 (S869) PTEN (S370)
BCL-XL (S62) Erk1/2 (Y204) NFkB-p105 (S927) PTEN (S380)
BCL-XL (T47) FADD (S194) NFkB-p105/p50 (S337) PTEN (S380/T382/T383)
BID (S78) FKHR (S256) NFkB-p105/p50 (S893) SAPK/JNK (T183)
BIM (S69/S65) FKHR (S319) NFkB-p105/p50 (S907) SAPK/JNK (Y185)
B-RAF (S446) FOXO1/3/4 pan (T24/T32) NFkB-p105/p50 (S932) Survivin (T117)
B-RAF (S601) FOXO1A (S329) NFkB-p65 (S276) TAK1 (T184)
B-RAF (T598) HSP90AB1 (S226) NFkB-p65 (S311) XIAP (S87)
CaMKII (T286) HSP27 (S15) NFkB-p65 (S468)

Protocol Outline

Our Analysis Service

For more detailed information about our antibody arrays, please feel free to contact us.

References

  1. Fulda, S.; et al. Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene. 2006, 25(34), 4798-4811.
  2. Xu, G.; et al. Apoptosis signaling pathways and lymphocyte homeostasis. Cell research. 2007, 17(9), 759-771.