Cytoskeleton Pathway Phosphorylation Antibody Array
Cytoskeleton is an interconnected assembly of actin, intermediate filament and microtubule network that extends throughout the entire cell. The cytoskeleton is involved in intracellular signal transduction such as regulation of cell shape, motility, transport and interactions with the environment. The cytoskeleton may affect signaling indirectly, for example, by regulating intracellular vesicle trafficking. Alternatively, physical contact between the cytoskeleton and signaling factors could modulate the spatiotemporal signaling output.
Fig.1 Organizational structures and physical interactions of actin, microtubules, and intermediate filaments.1
Different mechanisms underlie the cytoskeleton-dependent regulation of cell signaling. Changing the properties of the cytoskeleton provides ample opportunities for the fine-tuning of signaling events. Creative Biolabs offers cytoskeleton signal pathway phosphorylation screening service with antibody array, which consists of 141 high-specific antibodies and more than 70 phosphorylation sites involved in cytoskeleton signal pathways can be tested.
Related Signaling Pathway
| Signal Pathways | |
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Antibody Array Targets
| Targets |
| ACTIN a/b/g pan (Y55/Y53) | ERK8 (T175/Y177) | MEK1(S221) | PIP5K (S307) |
| Calmodulin (T79/S81) | Ezrin (T566) | MEK1(T291) | PKA CAT (T197) |
| CAMK1A (T177) | Ezrin (Y353) | MEK2 (T394) | PRKCA (Y657) |
| CAMK2 (T305) | Ezrin (Y478) | Merlin (S10) | PRKCA/PRKCB2 (T638) |
| CAMK2B/CAMK2G/CAMK2D (T287) | FAK (S910) | Merlin (S518) | PKC pan phospho-activation sites |
| CAMK4 (T196/T200) | FAK (Y397) | MKK3 (S189) | PLC-beta (S1105) |
| CAMK2 (T286) | FAK (Y407) | MKK3/MAP2K3 (T222) | PLCbeta3 (S537) |
| Cofilin (S3) | FAK (Y576) | MKK6 (S207) | Rac1/cdc42 (S71) |
| Cortactin (Y421) | FAK (Y861) | MKK7/MAP2K7 (S271) | ARHGEF2 (S885) |
| Cortactin (Y466) | FAK (Y925) | MKK7/MAP2K7 (T275) | Src (S75) |
| c-Raf (S296) | FLNA (S2152) | MYL2 (S18) | Src (Y216) |
| c-Raf (S43) | GRB2 (S159) | p130Cas (Y165) | Src (Y418) |
| CrkII (Y221) | GAP (S387) | p130Cas (Y410) | Src (Y529) |
| CrkL (Y207) | LIMK1 (T508) | Paxillin (Y118) | VASP (S157) |
| Erk1/2 (T202) | MEK1 (S298) | Paxillin (Y31) | VASP (S238) |
| Erk1/2 (Y204) | MEK1 (T286) | PIK3R1 (Y607) | WASP (Y290) |
| ERK3 (S189) | MEK1(S217) | PIK3R1/PIK3R3 (Y467/Y199) | WAVE1 (Y125) |
Protocol Outline
- Extracted protein and biotinylated
- Blocked antibody array
- Incubate with treated protein and control
- Incubate with streptavidin-conjugated fluorophore
- Scan with a microarray laser scanner
- Perform densitometry and analysis
Our Analysis Service
- Suitable for protein, cell and tissue samples from human, mouse and rat
- 6×107 cells or 150 µg protein
- Set replicate spots: treated sample and control
- Results are delivered in 4-5 weeks
For more detailed information about our antibody arrays, please feel free to contact us.
Reference
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Hohmann, Tim, and Faramarz Dehghani. "The cytoskeleton—a complex interacting meshwork." Cells 8.4 (2019): 362.
Distributed under Open Access license CC BY 4.0, without modification.