EGFR is a transmembrane protein belonging to the ErbB family of receptors, which functions as a receptor tyrosine kinase (RTK) in many cellular processes including cell growth, cell proliferation, cell survival and metastasis pathways. EGFR activity is regulated by common mutations, truncations to its extracellular domain, kinase domain mutation (L858R and T790M), or the exon 19 truncation[1]. EGFR is commonly upregulated in cancers such as non-small-cell lung cancer, metastatic colorectal cancer, glioblastoma, head and neck cancer, pancreatic cancer, and breast cancer.
Fig.1 Targeting of the EGFR signaling pathway[2].
Driven largely by its role in promoting cell proliferation and opposing apoptosis, the EGFR has been vilified as a proto-oncogene. Creative Biolabs offers EGFR signal pathway phosphorylation screening service with antibody array, which consists of 214 high-specific antibodies and more than 110 phosphorylation sites involved in EGFR signal pathways can be tested.
| Signal Pathways | |
|
|
| Targets |
| AKT (S473) | FAK (S910) | MEK1 (S298) | PLCG2 (Y753) |
| AKT (T308) | FAK (Y397) | MEK1 (T286) | Rac1/cdc42 (S71) |
| AKT (Y326) | FAK (Y407) | MEK1 (T291) | Raf1 (S259) |
| CTNNB1 (S33) | FAK (Y576) | MEK2 (T394) | Raf1 (S338) |
| CTNNB1 (S37) | FAK (Y861) | MAP2K3 (S189) | Raf1 (Y341) |
| CTNNB1 (T41/S45) | FAK (Y925) | MAP2K3 (T222) | Raf1(S621) |
| CTNNB1 (Y489) | Gab1 (Y627) | MAP2K6 (S207) | Ras-GRF1 (S916) |
| CTNNB1 (Y654) | Gab1 (Y659) | MAP2K7 (S271) | ARHGEF2 (S885) |
| CAV1 (Y14) | GRB2 (S159) | MAP2K7 (T275) | SAPK/JNK (T183) |
| CBL (Y700) | HER2 (Y1221/Y1222) | Erk1/2 (T202) | SAPK/JNK (Y185) |
| CBL (Y774) | HER2 (Y1248) | Erk1/2 (Y204) | SEK1/MKK4 (S80) |
| c-Raf (S296) | HER2 (Y877) | PAK1 (S204) | SEK1/MKK4 (T261) |
| c-Raf (S43) | IKBKG (S85) | PAK1 (T212) | SEK1/MKK4/JNKK1 (S257) |
| Dok-2 (Y299) | IKBKA (T23) | PAK1/2 (S199) | Shc (Y349) |
| EGFR (S1070) | IKBKA/IKBKB (S180/S181) | PAK1/2/3 (T423/T402/T421) | Shc (Y427) |
| EGFR (T678) | IKBKB (Y188) | Paxillin (Y118) | Src (S75) |
| EGFR (T693) | IKBKB (Y199) | Paxillin (Y31) | Src (Y216) |
| EGFR (Y1016) | IKBKG (S31) | PDK1 (S241) | Src (Y418) |
| EGFR (Y1069) | JAK1 (Y1022) | PIK3R1 (Y607) | Src (Y529) |
| EGFR (Y1092) | JAK2 (Y1007) | PIK3R1/PIK3R3 (Y467/Y199) | STAT1 (S727) |
| EGFR (Y1110) | JAK2 (Y221) | PKC phospho activation site | STAT1 (Y701) |
| EGFR (Y1172) | JNK1/2/3 (T183/Y185) | PLCG1 (Y1253) | STAT3 (S727) |
| EGFR (Y1197) | MAP3K1 (T1381) | PLCG1 (Y771) | STAT3 (Y705) |
| EGFR (Y869) | MEK1 (S217) | PLCG1 (Y783) | VAV1 (Y174) |
| ERK3 (S189) | MEK1 (S221) | PLCG2 (Y1217) | VAV2 (Y142) |
| ERK8 (T175/Y177) |
For more detailed information about our antibody arrays, please feel free to contact us.
References
