ATG14

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604). Modulates the hepatic lipid metabolism (By similarity).

Full Name

Autophagy Related 14

Function

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071).
Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597).
Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597).
Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393).
Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393).
Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604).
Modulates the hepatic lipid metabolism (By similarity).

Biological Process

Autophagosome assembly Source: UniProtKB
Autophagosome membrane docking Source: GO_Central
Cellular response to glucose starvation Source: UniProtKB
Cellular response to starvation Source: ParkinsonsUK-UCL
Endosome to lysosome transport Source: MGI
Macroautophagy Source: ParkinsonsUK-UCL
Mitophagy Source: ParkinsonsUK-UCL
Negative regulation of protein phosphorylation Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
Positive regulation of protein phosphorylation Source: UniProtKB
Posttranscriptional regulation of gene expression Source: ParkinsonsUK-UCL
Regulation of protein complex stability Source: ParkinsonsUK-UCL
Regulation of protein phosphorylation Source: ParkinsonsUK-UCL
Regulation of triglyceride metabolic process Source: Ensembl
Response to mitochondrial depolarisation Source: BHF-UCL

Cellular Location

Cytoplasm; Endoplasmic reticulum membrane; Preautophagosomal structure membrane; Autophagosome membrane. Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns3P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).