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Rabbit Anti-ATG14 Recombinant Antibody (10HCLC) (CBMAB-A3903-YC)

Provided herein is a Rabbit monoclonal antibody against Human Autophagy Related 14. The antibody can be used for immunoassay techniques, such as ICC, IF, WB.
See all ATG14 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
10HCLC
Antibody Isotype
IgG
Application
ICC, IF, WB

Basic Information

Immunogen
Multiple peptides corresponding to Human ATG14 (aa 63-79, 162-178, 277-293).
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1-2 µg/ml
IF(ICC)2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 14
Introduction
ATG14 is required for both basal and inducible autophagy. ATG14 determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. ATG14 plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine.
Entrez Gene ID
22863
UniProt ID
Q6ZNE5
Alternative Names
Autophagy Related 14; KIAA0831; Barkor; Beclin 1-Associated Autophagy-Related Key Regulator; Autophagy-Related Protein 14-Like Protein; ATG14L; ATG14 Autophagy Related 14 Homolog (S. Cerevisiae); ATG14 Autophagy Related 14 Homolog; Beclin 1-Interacting Pr
Function
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071).
Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597).
Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597).
Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393).
Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393).
Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604).
Modulates the hepatic lipid metabolism (By similarity).
Biological Process
Autophagosome assembly Source: UniProtKB
Autophagosome membrane docking Source: GO_Central
Cellular response to glucose starvation Source: UniProtKB
Cellular response to starvation Source: ParkinsonsUK-UCL
Endosome to lysosome transport Source: MGI
Macroautophagy Source: ParkinsonsUK-UCL
Mitophagy Source: ParkinsonsUK-UCL
Negative regulation of protein phosphorylation Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
Positive regulation of protein phosphorylation Source: UniProtKB
Posttranscriptional regulation of gene expression Source: ParkinsonsUK-UCL
Regulation of protein complex stability Source: ParkinsonsUK-UCL
Regulation of protein phosphorylation Source: ParkinsonsUK-UCL
Regulation of triglyceride metabolic process Source: Ensembl
Response to mitochondrial depolarisation Source: BHF-UCL
Cellular Location
Cytoplasm; Endoplasmic reticulum membrane; Preautophagosomal structure membrane; Autophagosome membrane. Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns3P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).

Zhao, Y., Zou, Z., Sun, D., Li, Y., Sinha, S. C., Yu, L., ... & Levine, B. (2021). GLIPR2 is a negative regulator of autophagy and the BECN1-ATG14-containing phosphatidylinositol 3-kinase complex. Autophagy, 1-14.

Hu, Z., Cai, M., Zhang, Y., Tao, L., & Guo, R. (2020). miR-29c-3p inhibits autophagy and cisplatin resistance in ovarian cancer by regulating FOXP1/ATG14 pathway. Cell Cycle, 19(2), 193-206.

Wang, Y., Zhang, C. X., Ge, S. L., & Gong, W. H. (2020). CTBP1‑AS2 inhibits proliferation and induces autophagy in ox‑LDL‑stimulated vascular smooth muscle cells by regulating miR‑195‑5p/ATG14. International Journal of Molecular Medicine, 46(2), 839-848.

Dong, Z., Xie, X., Sun, Y., Wu, H., & Dai, M. (2020). Paeonol prevents lipid metabolism dysfunction in palmitic acid-induced HepG2 injury through promoting SIRT1-FoxO1-ATG14-dependent autophagy. European journal of pharmacology, 880, 173145.

Park, J. M., Seo, M., Jung, C. H., Grunwald, D., Stone, M., Otto, N. M., ... & Kim, D. H. (2018). ULK1 phosphorylates Ser30 of BECN1 in association with ATG14 to stimulate autophagy induction. Autophagy, 14(4), 584-597.

Lu, D., Yang, C., Zhang, Z., Cong, Y., & Xiao, M. (2018). Knockdown of Linc00515 inhibits multiple myeloma autophagy and chemoresistance by upregulating miR-140-5p and downregulating ATG14. Cellular Physiology and Biochemistry, 48(6), 2517-2527.

Li, B., Wu, X., Chen, H., Zhuang, C., Zhang, Z., Yao, S., ... & Su, Q. (2018). miR199a-5p inhibits hepatic insulin sensitivity via suppression of ATG14-mediated autophagy. Cell death & disease, 9(3), 1-15.

Ozeki, N., Hase, N., Hiyama, T., Yamaguchi, H., Kawai-Asano, R., Nakata, K., & Mogi, M. (2017). RETRACTED: MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells.

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For research use only. Not intended for any clinical use.

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