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Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375)

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Tested Data
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in ELISA
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in ELISA
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in WB
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in WB
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in WB
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in WB
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in IHC
Mouse Anti-ATG14 Recombinant Antibody (CBT3478) (V2LY-0625-LY375) in IHC
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBT3478
Application
IHC
Immunogen
Purified recombinant fragment of human ATG14L (AA: 43-303) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Autophagy Related 14
Entrez Gene ID
22863
UniProt ID
Q6ZNE5
Function
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071).
Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597).
Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597).
Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393).
Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393).
Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604).
Modulates the hepatic lipid metabolism (By similarity).
Biological Process
Autophagosome assembly Source: UniProtKB
Autophagosome membrane docking Source: GO_Central
Cellular response to glucose starvation Source: UniProtKB
Cellular response to starvation Source: ParkinsonsUK-UCL
Endosome to lysosome transport Source: MGI
Macroautophagy Source: ParkinsonsUK-UCL
Mitophagy Source: ParkinsonsUK-UCL
Negative regulation of protein phosphorylation Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
Positive regulation of protein phosphorylation Source: UniProtKB
Posttranscriptional regulation of gene expression Source: ParkinsonsUK-UCL
Regulation of protein complex stability Source: ParkinsonsUK-UCL
Regulation of protein phosphorylation Source: ParkinsonsUK-UCL
Regulation of triglyceride metabolic process Source: Ensembl
Response to mitochondrial depolarisation Source: BHF-UCL
Cellular Location
Cytoplasm; Endoplasmic reticulum membrane; Preautophagosomal structure membrane; Autophagosome membrane. Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns3P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).

Zhao, Y., Zou, Z., Sun, D., Li, Y., Sinha, S. C., Yu, L., ... & Levine, B. (2021). GLIPR2 is a negative regulator of autophagy and the BECN1-ATG14-containing phosphatidylinositol 3-kinase complex. Autophagy, 1-14.

Hu, Z., Cai, M., Zhang, Y., Tao, L., & Guo, R. (2020). miR-29c-3p inhibits autophagy and cisplatin resistance in ovarian cancer by regulating FOXP1/ATG14 pathway. Cell Cycle, 19(2), 193-206.

Wang, Y., Zhang, C. X., Ge, S. L., & Gong, W. H. (2020). CTBP1‑AS2 inhibits proliferation and induces autophagy in ox‑LDL‑stimulated vascular smooth muscle cells by regulating miR‑195‑5p/ATG14. International Journal of Molecular Medicine, 46(2), 839-848.

Dong, Z., Xie, X., Sun, Y., Wu, H., & Dai, M. (2020). Paeonol prevents lipid metabolism dysfunction in palmitic acid-induced HepG2 injury through promoting SIRT1-FoxO1-ATG14-dependent autophagy. European journal of pharmacology, 880, 173145.

Park, J. M., Seo, M., Jung, C. H., Grunwald, D., Stone, M., Otto, N. M., ... & Kim, D. H. (2018). ULK1 phosphorylates Ser30 of BECN1 in association with ATG14 to stimulate autophagy induction. Autophagy, 14(4), 584-597.

Lu, D., Yang, C., Zhang, Z., Cong, Y., & Xiao, M. (2018). Knockdown of Linc00515 inhibits multiple myeloma autophagy and chemoresistance by upregulating miR-140-5p and downregulating ATG14. Cellular Physiology and Biochemistry, 48(6), 2517-2527.

Li, B., Wu, X., Chen, H., Zhuang, C., Zhang, Z., Yao, S., ... & Su, Q. (2018). miR199a-5p inhibits hepatic insulin sensitivity via suppression of ATG14-mediated autophagy. Cell death & disease, 9(3), 1-15.

Ozeki, N., Hase, N., Hiyama, T., Yamaguchi, H., Kawai-Asano, R., Nakata, K., & Mogi, M. (2017). RETRACTED: MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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