CAPN1

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Full Name

Calpain 1

Function

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed:21531719, PubMed:2400579).
Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed:23707407).

Biological Process

Cornification Source: Reactome
Extracellular matrix disassembly Source: Reactome
Mammary gland involution Source: Ensembl
Neutrophil degranulation Source: Reactome
Positive regulation of cell population proliferation Source: ProtInc
Proteolysis Source: UniProtKB
Receptor catabolic process Source: Ensembl
Regulation of catalytic activity Source: UniProtKB
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Regulation of NMDA receptor activity Source: ARUK-UCL
Self proteolysis Source: UniProtKB

Cellular Location

Cell membrane; Cytoplasm. Translocates to the plasma membrane upon Ca2+ binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).

Involvement in disease

Spastic paraplegia 76, autosomal recessive (SPG76): A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.

PTM

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.

Anti-CAPN1 antibodies

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Rabbit Anti-CAPN1 Recombinant Antibody (C1054) (CBMAB-C1054-CQ)

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Target: CAPN1

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat

Clone: C1054

Application*: WB, IF, E

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBYC-P034) (CBMAB-P0132-YC)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBYC-P034

Application*: E, P

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBYY-C0897) (CBMAB-C2334-YY)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBYY-C0897

Application*: P, IF, IP, WB

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBFYC-0791) (CBMAB-C0846-FY)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human, Mouse, Rat

Clone: CBFYC-0791

Application*: E, IF, IP, WB

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBFYC-0790) (CBMAB-C0845-FY)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG2a, κ

Specificity: Human, Mouse, Rat

Clone: CBFYC-0790

Application*: WB, IP

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBFYM-0894) (CBMAB-M1042-FY)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human, Mouse, Rat

Clone: CBFYM-0894

Application*: IF, IP, WB

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBFYM-0893) (CBMAB-M1041-FY)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Cattle, Dog, Human, Mouse, Pig, Horse, Rat

Clone: CBFYM-0893

Application*: E, IF, IP, WB

Datasheet SDS

Human CAPN1 ELISA Kit (V2LY-0626-LY4064)

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Target: CAPN1

Sensitivity: 0.022 ng/mL

Detection Range: 0.05-20 ng/mL

Sample Type: Serum, Plasma, cell culture supernates

Specificity: Human

Assay Type: Sandwich

Reactivity: Human

Datasheet SDS

Rat Capn1 ELISA Kit (2) (V2LY-0626-LY1913)

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Target: Capn1

Sensitivity: 0.049 ng/mL

Detection Range: 0.1-40 ng/mL

Sample Type: Serum, Plasma, cell culture supernates

Specificity: Rat

Assay Type: Sandwich

Reactivity: Rat

Datasheet SDS

Rat Capn1 ELISA Kit (V2LY-0626-LY1567)

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Target: Capn1

Sensitivity: 0.028 ng/mL

Detection Range: 0.05-30 ng/mL

Sample Type: Serum, Plasma, cell culture supernates

Specificity: Rat

Assay Type: Sandwich

Reactivity: Rat

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBT2753

Application*: F

Datasheet SDS

Mouse Anti-CAPN1 Recombinant Antibody (CBT4155) (V2LY-0625-LY1235)

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Target: CAPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBT4155

Application*: WB, IH, F

Datasheet SDS

More Infomation

Fig.3 Signaling pathways in cancers. (Creative Biolabs Authorized)

Fig.4 Protocols troubleshootings & guides. (Creative Biolabs Authorized)

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(P): Predicted

* Abbreviations

AActivation
AGAgonist
APApoptosis
BBlocking
BABioassay
BIBioimaging
CImmunohistochemistry-Frozen Sections
CIChromatin Immunoprecipitation
CTCytotoxicity
CSCostimulation
DDepletion
DBDot Blot

EELISA
ECELISA(Cap)
EDELISA(Det)
ESELISpot
EMElectron Microscopy
FFlow Cytometry
FNFunction Assay
GSGel Supershift
IInhibition
IAEnzyme Immunoassay
ICImmunocytochemistry
IDImmunodiffusion
IEImmunoelectrophoresis

IFImmunofluorescence
IGImmunochromatography
IHImmunohistochemistry
IMImmunomicroscopy
IOImmunoassay
IPImmunoprecipitation
ISIntracellular Staining for Flow Cytometry
LALuminex Assay
LFLateral Flow Immunoassay
MMicroarray
MCMass Cytometry/CyTOF
MDMeDIP

MSElectrophoretic Mobility Shift Assay
NNeutralization
PImmunohistologyp-Paraffin Sections
PAPeptide Array
PEPeptide ELISA
PLProximity Ligation Assay
RRadioimmunoassay
SStimulation
SESandwich ELISA
SHIn situ hybridization
TCTissue Culture
WBWestern Blot

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