Mouse Anti-CAPN1 Recombinant Antibody (CBFYM-0893) (CBMAB-M1041-FY)

Name: Mouse Anti-CAPN1 Recombinant Antibody (CBFYM-0893)
SKU: CBMAB-M1041-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CAPN1 Recombinant Antibody (CBFYM-0893) (CBMAB-M1041-FY) in IF

The TRPA1 blockers reduced the immunofluorescence intensity of NOX1 and Calpian1 on D3 in the ICH mice (n ≥ 3). ...View More

Xia, M., Chen, W., Wang, J., Yin, Y., Guo, C., Li, C., ... & Feng, H. (2019). TRPA1 activation-induced myelin degradation plays a key role in motor dysfunction after intracerebral hemorrhage. Frontiers in Molecular Neuroscience, 12, 98.

Mouse Anti-CAPN1 Recombinant Antibody (CBFYM-0893) (CBMAB-M1041-FY) in WB

NOX1 and Calpain1 expression were reduced in the ICH mice after application of the TRPA1 blockers. The sham group was assessed on D3 after sham surgery (n ≥ 3). Scale bars represent 50 μm (*P < 0.05 vs. sham group, **P < 0.01 vs. sham group, #P < 0.05, ##P < 0.01). ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0893

Application

ELISA, IF, IP, WB

Immunogen

Amino acids 682-709 of Calpain 1 catalytic subunit of human origin.

Host Species

Mouse

Specificity

Cattle, Dog, Human, Mouse, Pig, Horse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calpain 1

Introduction

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Entrez Gene ID

Human	823
Mouse	12333
Rat	29153
Cattle	281661
Pig	397027
Hamster	101834701
Rabbit	100009089

UniProt ID

Human	P07384
Mouse	O35350
Rat	P97571
Cattle	Q27970
Pig	P35750
Hamster	A0A1U8D291
Rabbit	P06815

Alternative Names

Calpain 1; Cell Proliferation-Inducing Gene 30 Protein; Calcium-Activated Neutral Proteinase 1; Calpain 1, (Mu/I) Large Subunit; Calpain-1 Large Subunit; Micromolar-Calpain; Calpain Mu-Type; EC 3.4.22.52; CANP 1; CANPL1

Function

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed:21531719, PubMed:2400579).
Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed:23707407).

Biological Process

Cornification Source: Reactome
Extracellular matrix disassembly Source: Reactome
Mammary gland involution Source: Ensembl
Neutrophil degranulation Source: Reactome
Positive regulation of cell population proliferation Source: ProtInc
Proteolysis Source: UniProtKB
Receptor catabolic process Source: Ensembl
Regulation of catalytic activity Source: UniProtKB
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Regulation of NMDA receptor activity Source: ARUK-UCL
Self proteolysis Source: UniProtKB

Cellular Location

Cell membrane; Cytoplasm. Translocates to the plasma membrane upon Ca2+ binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).

Involvement in disease

Spastic paraplegia 76, autosomal recessive (SPG76): A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.

PTM

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Su, W., Bi, X., Wang, Y., & Baudry, M. (2021). Changes in neurodegeneration-related miRNAs in brains from CAPN1−/− mice. BBA Advances, 1, 100004.

Su, W., Zhou, Q., Wang, Y., Chishti, A., Li, Q. Q., Dayal, S., ... & Baudry, M. (2020). Deletion of the Capn1 gene results in alterations in signaling pathways related to Alzheimer’s disease, protein quality control and synaptic plasticity in mouse brain. Frontiers in genetics, 11, 334.

Lai, L. L., Chen, Y. J., Li, Y. L., Lin, X. H., Wang, M. W., Dong, E. L., ... & Lin, X. (2020). Novel CAPN1 mutations extend the phenotypic heterogeneity in combined spastic paraplegia and ataxia. Annals of clinical and translational neurology, 7(10), 1862-1869.

Matos, A. M., Pinto, F. R., Barros, P., Amaral, M. D., Pepperkok, R., & Matos, P. (2019). Inhibition of calpain 1 restores plasma membrane stability to pharmacologically rescued Phe508del-CFTR variant. Journal of Biological Chemistry, 294(36), 13396-13410.

Cotti Piccinelli, S., Bassi, M. T., Citterio, A., Manganelli, F., Tozza, S., Santorelli, F. M., ... & Filosto, M. (2019). A Novel CAPN1 mutation causes a pure hereditary spastic paraplegia in an Italian family. Frontiers in neurology, 10, 580.

Peng, F., Sun, Y. M., Quan, C., Wang, J., & Wu, J. J. (2019). Two novel homozygous mutations of CAPN1 in Chinese patients with hereditary spastic paraplegia and literatures review. Orphanet journal of rare diseases, 14(1), 1-9.

Kocoglu, C., Gundogdu, A., Kocaman, G., Kahraman-Koytak, P., Uluc, K., Kiziltan, G., ... & Basak, A. N. (2018). Homozygous CAPN1 mutations causing a spastic-ataxia phenotype in 2 families. Neurology Genetics, 4(1).

Liu, B., Zhou, Y., Lu, D., Liu, Y., Zhang, S. Q., Xu, Y., ... & Gu, X. (2017). Comparison of the protein expression of calpain‑1, calpain‑2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa. Oncology letters, 14(3), 3705-3710.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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