Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270)


See all CAPN1 antibodies
Online Inquiry
Tested Data
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in ELISA
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in ELISA
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in WB
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in WB
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in WB
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in WB
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in FC
Mouse Anti-CAPN1 Recombinant Antibody (CBT2753) (V2LY-0625-LY1270) in FC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2753
Antibody Isotype
IgG1
Application
FC

Basic Information

Immunogen
Purified recombinant fragment of human CAPN1 (AA: 501-714) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
823
UniProt ID
P07384
Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed:21531719, PubMed:2400579).
Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed:23707407).
Biological Process
Cornification Source: Reactome
Extracellular matrix disassembly Source: Reactome
Mammary gland involution Source: Ensembl
Neutrophil degranulation Source: Reactome
Positive regulation of cell population proliferation Source: ProtInc
Proteolysis Source: UniProtKB
Receptor catabolic process Source: Ensembl
Regulation of catalytic activity Source: UniProtKB
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Regulation of NMDA receptor activity Source: ARUK-UCL
Self proteolysis Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm. Translocates to the plasma membrane upon Ca2+ binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).
Involvement in disease
Spastic paraplegia 76, autosomal recessive (SPG76): A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
PTM
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
More Infomation

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Su, W., Bi, X., Wang, Y., & Baudry, M. (2021). Changes in neurodegeneration-related miRNAs in brains from CAPN1−/− mice. BBA Advances, 1, 100004.

Su, W., Zhou, Q., Wang, Y., Chishti, A., Li, Q. Q., Dayal, S., ... & Baudry, M. (2020). Deletion of the Capn1 gene results in alterations in signaling pathways related to Alzheimer’s disease, protein quality control and synaptic plasticity in mouse brain. Frontiers in genetics, 11, 334.

Lai, L. L., Chen, Y. J., Li, Y. L., Lin, X. H., Wang, M. W., Dong, E. L., ... & Lin, X. (2020). Novel CAPN1 mutations extend the phenotypic heterogeneity in combined spastic paraplegia and ataxia. Annals of clinical and translational neurology, 7(10), 1862-1869.

Matos, A. M., Pinto, F. R., Barros, P., Amaral, M. D., Pepperkok, R., & Matos, P. (2019). Inhibition of calpain 1 restores plasma membrane stability to pharmacologically rescued Phe508del-CFTR variant. Journal of Biological Chemistry, 294(36), 13396-13410.

Cotti Piccinelli, S., Bassi, M. T., Citterio, A., Manganelli, F., Tozza, S., Santorelli, F. M., ... & Filosto, M. (2019). A Novel CAPN1 mutation causes a pure hereditary spastic paraplegia in an Italian family. Frontiers in neurology, 10, 580.

Peng, F., Sun, Y. M., Quan, C., Wang, J., & Wu, J. J. (2019). Two novel homozygous mutations of CAPN1 in Chinese patients with hereditary spastic paraplegia and literatures review. Orphanet journal of rare diseases, 14(1), 1-9.

Kocoglu, C., Gundogdu, A., Kocaman, G., Kahraman-Koytak, P., Uluc, K., Kiziltan, G., ... & Basak, A. N. (2018). Homozygous CAPN1 mutations causing a spastic-ataxia phenotype in 2 families. Neurology Genetics, 4(1).

Liu, B., Zhou, Y., Lu, D., Liu, Y., Zhang, S. Q., Xu, Y., ... & Gu, X. (2017). Comparison of the protein expression of calpain‑1, calpain‑2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa. Oncology letters, 14(3), 3705-3710.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CAPN1 Recombinant Antibody (CBT2753)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry