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CD3E

CD3E (CD3e Molecule) is a Protein Coding gene. Diseases associated with CD3E include Immunodeficiency 18 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are G-protein signaling N-RAS regulation pathway and Toll-like receptor signaling pathway. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and signal transducer activity.
Full Name
CD3e Molecule
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Apoptotic signaling pathway Source: Ensembl
Cell surface receptor signaling pathway Source: GO_Central
Cerebellum development Source: Ensembl
Dendrite development Source: Ensembl
G protein-coupled receptor signaling pathway Source: ProtInc
Negative regulation of gene expression Source: UniProtKB
Negative regulation of smoothened signaling pathway Source: Ensembl
Negative thymic T cell selection Source: Ensembl
Positive regulation of alpha-beta T cell proliferation Source: Ensembl
Positive regulation of calcium-mediated signaling Source: Ensembl
Positive regulation of cell-cell adhesion mediated by integrin Source: BHF-UCL
Positive regulation of cell-matrix adhesion Source: BHF-UCL
Positive regulation of gene expression Source: UniProtKB
Positive regulation of interferon-gamma production Source: Ensembl
Positive regulation of interleukin-2 production Source: Ensembl
Positive regulation of interleukin-4 production Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
Positive regulation of T cell anergy Source: Ensembl
Positive regulation of T cell proliferation Source: UniProtKB
Positive thymic T cell selection Source: GO_Central
Protein-containing complex assembly Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of immune response Source: Reactome
Response to nutrient Source: Ensembl
Signal complex assembly Source: ProtInc
T cell activation Source: UniProtKB
T cell costimulation Source: Ensembl
T cell receptor signaling pathway Source: Reactome
Transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc
Cellular Location
Cell membrane
Involvement in disease
Immunodeficiency 18 (IMD18): An autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood of recurrent infections. The severity is variable, encompassing both a mild immunodeficiency and severe combined immunodeficiency (SCID), resulting in early death without bone marrow transplantation in some patients. Immunologic work-up of the IMD18 SCID patients shows a T cell-negative, B cell-positive, natural killer (NK) cell-positive phenotype, whereas T-cell development is not impaired in the mild form of IMD18.
Topology
Extracellular: 23-126
Helical: 127-152
Cytoplasmic: 153-207
PTM
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Anti-CD3E antibodies

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Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYC-1271
Application*: IH, F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBFYC-1269
Application*: E
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b
Specificity: Mouse
Clone: CBYY-C1356
Application*: F, IH, P, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBYY-C1355
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Chicken
Clone: CBYY-C0429
Application*: F, IC, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Pig
Clone: 5K105
Application*: IP, F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYY-C0394
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: CBYY-C0388
Application*: E, F, IF, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 4D10A6
Application*: E, WB, P, IF, F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 400
Application*: E, WB, P, IF, F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBYY-C0180
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Monkey
Clone: CBYY-C0179
Application*: F, IH
Target: CD3E
Host: Rat
Antibody Isotype: IgG2a
Specificity: Mouse
Clone: CBYY-C0177
Application*: F, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 3H3
Application*: WB, E
Target: CD3E
Host: Mouse
Specificity: Human
Clone: CBFYC-1278
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Dog
Clone: CBFYC-1277
Application*: F, IF, IH, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYC-1275
Application*: E
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBFYC-1274
Application*: WB, P, C
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Pig
Clone: 5K104
Application*: F, IH, IP
Target: CD3E
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: 2D37
Application*: WB, F
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b
Specificity: Mouse
Clone: 13H134
Application*: F, IH, IP
Target: CD3E
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: 500A2
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Pig
Clone: CBFYC-1267
Application*: F, IH
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b
Specificity: Mouse
Clone: CBFYC-1266
Application*: F, IH
Target: CD3E
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBFYC-1265
Application*: F
Target: CD3E
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBFYC-1264
Application*: F, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Pig
Clone: CBFYC-1263
Application*: F
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b
Specificity: Mouse
Clone: CBFYC-1262
Application*: F, IH, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBFYC-1261
Application*: F, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYC-0531
Application*: F, IH
Target: CD3E
Host: Armenian Hamster
Antibody Isotype: IgG1, κ
Specificity: Mouse
Clone: CBFYC-0288
Application*: F, C, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human, Mouse
Clone: CBFYC-0257
Application*: F, IF
Target: CD3E
Host: Rat
Antibody Isotype: IgG1
Specificity: Cattle, Dog, Chicken, Horse, Cat, Human, Mouse, Pig, Rabbit
Clone: CBFYC-0160
Application*: F, IF, IH, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBFYC-0047
Application*: F
Target: CD3E
Host: Armenian Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBFYC-0002
Application*: F, IH, IF, IP, WB
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b, κ
Specificity: Mouse
Clone: CBFYC-0001
Application*: F, IH, IP
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBAb056
Application*: WB, IH, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 3E12
Application*: WB, E
Target: CD3E
Host: Hamster
Antibody Isotype: IgG, κ
Specificity: Mouse
Clone: 5K3
Application*: F, IP
Target: CD3E
Host: Hamster
Antibody Isotype: IgG1
Specificity: Mouse
Clone: MAB0809
Application*: D, A
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b
Specificity: Mouse
Clone: MAB0808
Application*: D, A
Target: CD3E
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBFYM-0975
Application*: C, IF, F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: A051
Application*: FC, IHC
Target: CD3E
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: A050
Application*: FC, IF, IHC, IP, WB
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CAP400
Application*: FC, IHC, WB
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CAP399
Application*: FC, IHC, IP, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CAP398
Application*: ELISA, FC, IF
Target: CD3E
Host: Human
Antibody Isotype: IgG1
Specificity: Human
Clone: UCHT1
Application*: FC, IHC
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse
Clone: 500A2
Application*: FC
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Mouse
Clone: 500A2
Application*: FC
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 101
Application*: ELISA, IHC, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: F7.2.38
Application*: P
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CA-3
Application*: IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 8J32
Application*: E
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CF077
Application*: ELISA, WB, IHC
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: RM344
Application*: IHC, WB
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CB-298-5D12
Application*: WB, IP, IHC, ICC, IHC-IF, F, mIF
Target: CD3E
Host: Hamster
Antibody Isotype: IgG
Specificity: Mouse
Clone: SPM381
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: SPV-T3b
Application*: WB, C, F
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b
Specificity: Mouse
Clone: RM0027-3B19
Application*: IP, F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: MM0167-3F43
Application*: C, F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Pig
Clone: 13H137
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: UCHT1R
Application*: F
Target: CD3E
Host: Rat
Antibody Isotype: IgG2b
Specificity: Mouse
Clone: 17A2
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG3, κ
Specificity: Rat
Clone: G4.18
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Pig
Clone: BB23-8E6
Application*: F
Target: CD3E
Host: Rat
Antibody Isotype: IgG, κ
Specificity: Mouse
Clone: YCD3-1
Application*: F
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: RBT-CD3e
Application*: C, P
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: S4.1
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Pig
Clone: PPT3
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG3, λ
Specificity: Monkey
Clone: SP34
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Pig
Clone: 13H138
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: 5H55
Application*: FC, IF, IP, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 5F4
Application*: FC, IF
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 10K135
Application*: IF, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 13B131
Application*: E, FC, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 13H118
Application*: FC, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 13H114
Application*: FC, IH, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 001
Application*: F
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 0.N.47
Application*: WB, P, C
Target: CD3E
Host: rabbit
Antibody Isotype: IgG
Specificity: Human, Dog, Horse, Ferret, Monkey
Clone: 14B9
Application*: E, C, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 1A7E5G5
Application*: E
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: 301
Application*: E
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 3F43
Application*: F, IH, A
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 3H5
Application*: E, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 4C1
Application*: E, PL, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 4E2
Application*: E, F, WB
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: 801
Application*: WB, IP
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: 822
Application*: E
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 2Q1165
Application*: IF, IH, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 3H701
Application*: FC, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG3
Specificity: Human, Mouse, Rat
Clone: 5B2
Application*: E, FC, IF, IH, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 5B3
Application*: FC
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 5F3
Application*: FC, IF, IH
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 6D14
Application*: FC, IP
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 7H222
Application*: FC, IH, WB
Target: CD3E
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: M2AB
Application*: FC
Target: CD3E
Host: Rabbit
Specificity: Human
Clone: N26-R
Application*: IH
Target: CD3E
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: SP162
Application*: FC, WB, P
Target: CD3E
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 13H111
Application*: E
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For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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