Mouse Anti-CD3E Recombinant Antibody (CBT4396) (V2LY-0625-LY2399)

Name: Mouse Anti-CD3E Recombinant Antibody (CBT4396)
SKU: V2LY-0625-LY2399
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CD3E Recombinant Antibody (CBT4396) (V2LY-0625-LY2399) in WB

Mouse Anti-CD3E Recombinant Antibody (CBT4396) (V2LY-0625-LY2399) in FC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4396

Application

WB, FC

Immunogen

Purified recombinant fragment of CD3E expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD3e Molecule

Entrez Gene ID

916

UniProt ID

P07766

Alternative Names

CD3e Molecule; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3;

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Apoptotic signaling pathway Source: Ensembl
Cell surface receptor signaling pathway Source: GO_Central
Cerebellum development Source: Ensembl
Dendrite development Source: Ensembl
G protein-coupled receptor signaling pathway Source: ProtInc
Negative regulation of gene expression Source: UniProtKB
Negative regulation of smoothened signaling pathway Source: Ensembl
Negative thymic T cell selection Source: Ensembl
Positive regulation of alpha-beta T cell proliferation Source: Ensembl
Positive regulation of calcium-mediated signaling Source: Ensembl
Positive regulation of cell-cell adhesion mediated by integrin Source: BHF-UCL
Positive regulation of cell-matrix adhesion Source: BHF-UCL
Positive regulation of gene expression Source: UniProtKB
Positive regulation of interferon-gamma production Source: Ensembl
Positive regulation of interleukin-2 production Source: Ensembl
Positive regulation of interleukin-4 production Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
Positive regulation of T cell anergy Source: Ensembl
Positive regulation of T cell proliferation Source: UniProtKB
Positive thymic T cell selection Source: GO_Central
Protein-containing complex assembly Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of immune response Source: Reactome
Response to nutrient Source: Ensembl
Signal complex assembly Source: ProtInc
T cell activation Source: UniProtKB
T cell costimulation Source: Ensembl
T cell receptor signaling pathway Source: Reactome
Transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc

Cellular Location

Cell membrane

Involvement in disease

Immunodeficiency 18 (IMD18): An autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood of recurrent infections. The severity is variable, encompassing both a mild immunodeficiency and severe combined immunodeficiency (SCID), resulting in early death without bone marrow transplantation in some patients. Immunologic work-up of the IMD18 SCID patients shows a T cell-negative, B cell-positive, natural killer (NK) cell-positive phenotype, whereas T-cell development is not impaired in the mild form of IMD18.

Topology

Extracellular: 23-126
Helical: 127-152
Cytoplasmic: 153-207

PTM

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Liu, Y., Wu, Y., Zhang, P., Xu, C., Liu, Z., He, C., ... & Kang, Z. (2021). CXCL12 and CD3E as Indicators for Tumor Microenvironment Modulation in Bladder Cancer and Their Correlations With Immune Infiltration and Molecular Subtypes. Frontiers in Oncology, 11.

Mamoor, S. (2020). CD3E is differentially expressed in metastatic breast cancer, both in metastases to the brain and to the lymph nodes.

Shiraiwa, H., Narita, A., Kamata-Sakurai, M., Ishiguro, T., Sano, Y., Hironiwa, N., ... & Igawa, T. (2019). Engineering a bispecific antibody with a common light chain: Identification and optimization of an anti-CD3 epsilon and anti-GPC3 bispecific antibody, ERY974. Methods, 154, 10-20.

Miyazawa, R., Murata, N., Matsuura, Y., Shibasaki, Y., Yabu, T., & Nakanishi, T. (2018). Peculiar expression of CD3-epsilon in kidney of ginbuna crucian carp. Frontiers in immunology, 9, 1321.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CD3E Matched Antibody Pair (220) (CAT#: APMAB-220LY)

CD3E Matched Antibody Pair (221) (CAT#: APMAB-221LY)

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Isotype control

For research use only. Not intended for any clinical use.

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