TSG101

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq]

tumor susceptibility gene 101

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).

Biological Process autophagosome maturation Source:ParkinsonsUK-UCL1 Publication
Biological Process cell cycle Source:UniProtKB-KW
Biological Process cell division Source:UniProtKB-KW
Biological Process endosome to lysosome transport Source:GO_Central1 Publication
Biological Process exosomal secretion Source:Ensembl
Biological Process extracellular transport Source:UniProtKB1 Publication
Biological Process keratinocyte differentiation Source:Ensembl
Biological Process macroautophagy Source:ParkinsonsUK-UCL1 Publication
Biological Process membrane fission Source:ComplexPortal1 Publication
Biological Process multivesicular body assembly Source:ParkinsonsUK-UCL1 Publication
Biological Process negative regulation of cell population proliferation Source:Ensembl
Biological Process negative regulation of epidermal growth factor receptor signaling pathway Source:ParkinsonsUK-UCL1 Publication
Biological Process negative regulation of epidermal growth factor-activated receptor activity Source:UniProtKB1 Publication
Biological Process negative regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process positive regulation of exosomal secretion Source:UniProtKB2 Publications
Biological Process positive regulation of ubiquitin-dependent endocytosis Source:Ensembl
Biological Process positive regulation of viral budding via host ESCRT complex Source:UniProtKB2 Publications
Biological Process protein monoubiquitination Source:Ensembl
Biological Process protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source:ComplexPortal1 Publication
Biological Process regulation of cell cycle Source:Ensembl
Biological Process regulation of cell growth Source:Ensembl
Biological Process regulation of extracellular exosome assembly Source:UniProtKB1 Publication
Biological Process regulation of MAP kinase activity Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source:HGNC-UCL1 Publication
Biological Process viral budding Source:UniProtKB1 Publication
Biological Process viral budding via host ESCRT complex Source:ParkinsonsUK-UCL1 Publication
Biological Process viral release from host cell Source:UniProtKB2 Publications

Cytoplasm
Early endosome membrane
Late endosome membrane
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Midbody, Midbody ring
Nucleus
Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis.

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.