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Mouse Anti-Tsg101 Recombinant Antibody (CBYJT-5007) (CBMAB-T4564-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Tsg101 (Tumor Susceptibility Gene 101). The antibody can be used for immunoassay techniques, such as IP, WB.
See all Tsg101 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse
Clone
CBYJT-5007
Antibody Isotype
IgG1
Application
IP, WB

Basic Information

Specificity
Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 167-374

Target

Full Name
tumor susceptibility gene 101
Introduction
Tsg101 is a component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Tsg101 mediates the association between the ESCRT-0 and ESCRT-I complex. Tsg101 is required for completion of cytokinesis; the function requires CEP55. Tsg101 may be involved in cell growth and differentiation. acts as a negative growth regulator. Tsg101 is required for the exosomal release of SDCBP, CD63 and syndecan (By similarity). It may also function in the extracellular release of microvesicles that differ from the exosomes (By similarity).
Entrez Gene ID
22088
UniProt ID
Q61187
Alternative Names
CC2; AI255943
Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).
Biological Process
Biological Process autophagosome maturation Source:ParkinsonsUK-UCL1 Publication
Biological Process cell cycle Source:UniProtKB-KW
Biological Process cell division Source:UniProtKB-KW
Biological Process endosome to lysosome transport Source:GO_Central1 Publication
Biological Process exosomal secretion Source:Ensembl
Biological Process extracellular transport Source:UniProtKB1 Publication
Biological Process keratinocyte differentiation Source:Ensembl
Biological Process macroautophagy Source:ParkinsonsUK-UCL1 Publication
Biological Process membrane fission Source:ComplexPortal1 Publication
Biological Process multivesicular body assembly Source:ParkinsonsUK-UCL1 Publication
Biological Process negative regulation of cell population proliferation Source:Ensembl
Biological Process negative regulation of epidermal growth factor receptor signaling pathway Source:ParkinsonsUK-UCL1 Publication
Biological Process negative regulation of epidermal growth factor-activated receptor activity Source:UniProtKB1 Publication
Biological Process negative regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process positive regulation of exosomal secretion Source:UniProtKB2 Publications
Biological Process positive regulation of ubiquitin-dependent endocytosis Source:Ensembl
Biological Process positive regulation of viral budding via host ESCRT complex Source:UniProtKB2 Publications
Biological Process protein monoubiquitination Source:Ensembl
Biological Process protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source:ComplexPortal1 Publication
Biological Process regulation of cell cycle Source:Ensembl
Biological Process regulation of cell growth Source:Ensembl
Biological Process regulation of extracellular exosome assembly Source:UniProtKB1 Publication
Biological Process regulation of MAP kinase activity Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source:HGNC-UCL1 Publication
Biological Process viral budding Source:UniProtKB1 Publication
Biological Process viral budding via host ESCRT complex Source:ParkinsonsUK-UCL1 Publication
Biological Process viral release from host cell Source:UniProtKB2 Publications
Cellular Location
Cytoplasm
Early endosome membrane
Late endosome membrane
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Midbody, Midbody ring
Nucleus
Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis.
PTM
Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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