ERK Signal Pathway Phosphorylation Antibody Array
Extracellular signal-regulated kinase 1/2 (ERK) belongs to the mitogen-activated protein kinase (MAPK) family. ERK plays a role in signaling cascades and transmits extracellular signals to intracellular targets. Concerning processes downstream of ERK activation, the role of the ERK/MAPK signaling pathway in tumor extracellular matrix degradation and tumor angiogenesis is emphasized, and the role of ERK in tumor proliferation, invasion and metastasis is highlighted.
Fig.1 The ERK signaling pathway1.
The ERK signaling pathway is one of the most important and intensively studied signaling pathways, which functions in cellular proliferation, differentiation, and survival. Creative Biolabs offers ERK Signal pathway phosphorylation screening service with antibody array, which consists of 227 high-specific antibodies and more than 110 phosphorylation sites involved in ERK signal pathways can be tested.
Related Signaling Pathway
| Signal Pathways | |
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Antibody Array Targets
| Targets |
| A-RAF (Y301/Y302) | FosB (S27) | Myc (T58) | PLCG1 (Y771) |
| ARRB1 (S412) | GRB2 (S159) | Erk1/2 (T202) | PLCG1 (Y783) |
| BAD (S112) | GAP (S387) | Erk1/2 (T202/Y204) | PLCG2 (Y1217) |
| BAD (S134) | Histone H3.1 (S10) | Erk1/2 (Y204) | PLCG2 (Y753) |
| BAD (S136) | NFKBIA (S32/S36) | PAK1 (S204) | PP2A-a (Y307) |
| BAD (S155) | NFKBIA (Y42) | PAK1 (T212) | Pyk2 (Y402) |
| BAD (S91/S128) | IKKA (T23) | PAK1/2 (S199) | Pyk2 (Y579) |
| B-RAF (S446) | IKKA/IKKB (S180/S181) | PAK1/2/3 (S141) | Pyk2 (Y580) |
| B-RAF (S601) | MEK1 (S217) | PAK1/2/3 (T423/T402/T421) | Pyk2 (Y881) |
| B-RAF (T598) | MEK1 (S221) | PAK2 (S192) | Rac1/cdc42 (S71) |
| CAMK1A (T177) | MEK1 (S298) | PAK3 (S154) | Raf1 (S259) |
| CAMK2B/CAMK2G/CAMK2D (T287) | MEK1 (T286) | PIK3R1 (Y607) | Raf1 (S338) |
| CAMK4 (T196/T200) | MEK1 (T291) | PIK3R1/PIK3R3 (Y467/Y199) | Raf1 (Y341) |
| CAMK2 (T286) | MEK2 (T394) | PKA CAT (T197) | Raf1(S621) |
| c-Raf (S296) | MAP2K3 (S189) | PRKCA (Y657) | RapGEF1 (Y504) |
| c-Raf (S43) | MAP2K3 (T222) | PRKCA/PRKCB2 (T638) | Ras-GRF1 (S916) |
| CREB (S121) | MAP2K6 (S207) | PRKCB (S661) | ARHGEF2 (S885) |
| CREB (S129) | MAP2K7 (S271) | PRKCD (S645) | RSK1/2/3/4 (S221/S227/S218/S232) |
| CREB (S133) | MAP2K7 (T275) | PRKCD (T505) | Src (S75) |
| CREB (S142) | Mnk1 (T385) | PRKCE (S729) | Src (Y418) |
| CREB (T100) | MSK1 (S212) | PRKCQ (S676) | Src (Y529) |
| DYN1 (S774) | MSK1 (S360) | PRKCQ (T538) | SRF (S77) |
| Elk1 (S383) | MSK1 (S376) | PRKCZ (T410) | SRF (S99) |
| Elk1 (S389) | MSK1 (T581) | PRKCZ (T560) | STAT1 (S727) |
| Elk1 (T417) | MSK2 (T568) | PKR (T446) | STAT1 (Y701) |
| ERK3 (S189) | Myc (S373) | PKR (T451) | STAT3 (S727) |
| ERK8 (T175/Y177) | Myc (S62) | PLCB (S1105) | STAT3 (Y705) |
| Fos (T232) | Myc (T358) | PLCB3 (S537) |
Protocol Outline
- Extracted protein and biotinylated
- Blocked antibody array
- Incubate with treated protein and control
- Incubate with streptavidin-conjugated fluorophore
- Scan with a microarray laser scanner
- Perform densitometry and analysis
Our Analysis Service
- Suitable for protein, cell and tissue samples from human, mouse and rat
- 6×107 cells or 150 µg protein
- Set replicate spots: treated sample and control
- Results are delivered in 4-5 weeks
For more detailed information about our antibody arrays, please feel free to contact us.
Reference
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Arkun, Yaman, and Mohammadreza Yasemi. "Dynamics and control of the ERK signaling pathway: Sensitivity, bistability, and oscillations." PloS one 13.4 (2018): e0195513.
Distributed under Open Access license CC BY 4.0, without modification.