FAQs (Frequently asked questions) about our antibody products to answer customers commonly frequently asked technical questions.
We offer all kinds of antibody products including polyclonal antibodies, monoclonal antibodies, tag/control antibodies, secondary antibodies, recombinant antibodies, small molecules antibodies, ChIP-grade antibodies and antibody pairs. Our antibody-related services include custom recombinant antibody production, custom monoclonal antibody production, custom polyclonal antibody production and custom modified antibody production.
Creative Biolabs is a leader in the field of antibody development committed to developing various antibodies to meet every customer all over the world. Our antibodies are specific to a variety of species and can be used in multiple applications. Furthermore, we develop thousands of new antibody products every year. We have first-class technology platforms and professional technical team to meet high quality antibody production. We are confident to offer you the best antibodies to meet your requirements.
Generally, monoclonal antibodies are produced using in vivo ascites. For polyclonal antibodies, we use serum production. Additionally, we also use phage display technology to produce monoclonal antibodies.
Monoclonal antibodies are produced by identical immune cells that are clones of a specific parent cell. Monoclonal antibodies can only recognize the same antigen and epitope. Polyclonal antibodies are produced by different immune cells that have affinity for different epitopes of the same antigen. Therefore, monoclonal antibodies are much more specific and with less background noise than polyclonal antibodies. However, monoclonal antibodies are also usually more expensive to produce, less robust and are very sensitive to small changes in the antigen compared with polyclonal antibodies.
Primary antibody is used to recognize and bind to the target antigen, while the secondary antibody is used to target the primary antibody and often is labeled with an enzyme or dye that can be amplified for detection. Selecting a suitable secondary antibody depends on the following elements:
Our antibodies are recommended to use in WB with a dilution range of 1:500-1:2000. However, for ELISA assay, the optimal dilution should be determined by actual experiment and based on customer's assay platform and system.
Western blotting is a technique that separates proteins based on different protein size. However, the migration of proteins can be affected by many factors, which may cause the result that the actual band size observed may differ from that predicted. Generally, influence factors include:
Our immunogens include recombinant protein, native protein, peptide and other antigens. For the immunogen sequence, please refer to the product information on our website or product data sheet. Besides, you also can contact our technical support to request this information you needed.
Commonly, the store buffer of our antibodies contains 0.01M PBS (pH 7.4) with 50% glycerol. Sometimes other form buffers are also used in antibody storage based on different requirements. Detailed information can be found on our website or product data sheet.
Most of our antibodies are IgG isotype with a molecular weight of approximately 150 kDa.
For more information, please contact us.
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