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Lysate FAQs

FAQs (Frequently asked questions) at Creative Biolabs to answer your general frequently asked technical questions about lysate. Based on the extensive experience and advanced technical platforms, we will provide you with professional technical guidance.

  1. What is a lysate?

    2. Lysate is a sample of cells that have been lysed through cell membrane broken to release the contents.

  2. Why the recombinant or lysates molecular weights are different when using different antibodies?

    3. A variety of factors can affect the sizes of recombinant proteins or lysates due to Western blotting is a technique that separates proteins based upon different protein size. Normally, influence factors include:

    • The lysate or protein isn't full-length.
    • Post-translational modification, such as phosphorylation, glycosylation.
    • Splice variants. Alternative splicing often can create different sized proteins.
    • Relative charge between different amino acids.
    • Multimers.
  3. What is an overexpression lysate?

    4. An overexpression lysate is a lysate with a high concentration of a particular protein, which is often used as positive controls.

  4. Do I need to remove cell debris in the cell lysate before assaying for luciferase activity?

    5. For luciferase activity measure, it is not necessary to remove cell debris.

  5. What experiments can cell lysates be used for?

    6. Cell lysates usually can be used in Western blot, ELISA and a series of bio-analytical assays.

  6. Do you provide custom cell lysate service?

    7. Yes, we can provide Custom Cell Lysate Service to meet your scientific research needs. We have a proprietary platform for cell lysate preparation that can make your cell lysates at very high concentration of up to 20 mg/mL with affordable prices. In addition, scale-up production is also available.

  7. What is the optimal source for over-expression cell lysates?

    8. Usually, over-expression cell lysates are prepared from HEK293T cells. Each expressed protein has a C-terminal tag which can be detected by anti-tag antibodies for expression verification.

  8. How long can I store lysates?

    9. Lysates often can be stable for 12 months when are stored at -20℃. Importantly, it is better to avoid repeated freeze-thaw cycles, although some lysates in RIPA buffer will not cause significant protein degradation within 5 freeze-thaw cycles.

  9. Why sometimes I see more than one bands with some lysates in Western blot experiments?

    10. This result could be caused by post-translation modifications of protein, protein degradation, etc.

If you still have some other questions about the lysate, please do not hesitate to contact us.

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