Mouse Anti-CACNA1F Recombinant Antibody (1H6) (CBMAB-A1023-LY)

Name: Mouse Anti-CACNA1F Recombinant Antibody (1H6)
SKU: CBMAB-A1023-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CACNA1F Recombinant Antibody (1H6) (CBMAB-A1023-LY) in WB

CaVprotein expression was determined in three independently produced cell lysates (L1, L2 and L3) with specific antibodies by Western blot. ...View More

Pathe-Neuschäfer-Rube, A., Neuschäfer-Rube, F., & Püschel, G. P. (2021). Cell-based reporter release assay to determine the activity of calcium-dependent neurotoxins and neuroactive pharmaceuticals. Toxins, 13(4), 247.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1H6

Application

WB, IP, E

Immunogen

Amino acids 1878-1977 representing partial length Ca++ CP α1F 1F of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

calcium channel, voltage-dependent, L type, alpha 1F subunit

Introduction

This gene encodes a member of the alpha-1 subunit family; a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. There are multiple isoforms of each of the proteins in the complex, either encoded by different genes or the result of alternative splicing of transcripts. Alternate transcriptional splice variants of the gene described here have been observed but have not been thoroughly characterized. Mutations in this gene have been shown to cause incomplete X-linked congential stationary night blindness type 2 (CSNB2). [provided by RefSeq]

Entrez Gene ID

778

UniProt ID

O60840

Alternative Names

AIED; COD3; CORDX; CORDX3; CSNB2; CSNB2A; CSNBX2; Cav1.4; JM8; JMC8; OA2

Function

Isoform 1: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines. Activates at more negative voltages and does not undergo calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarization.
Isoform 4: Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exhibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations.
Isoform 6: Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations.

Biological Process

Calcium ion import Source: GO_Central
Calcium ion transport Source: GO_Central
Detection of light stimulus involved in visual perception Source: UniProtKB
Negative regulation of voltage-gated calcium channel activity Source: UniProtKB
Regulation of ion transmembrane transport Source: UniProtKB-KW
Visual perception Source: UniProtKB

Cellular Location

Membrane

Involvement in disease

Night blindness, congenital stationary, 2A (CSNB2A): A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia.
Cone-rod dystrophy, X-linked 3 (CORDX3): An inherited retinal dystrophy characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa, due to cone photoreceptors degenerating at a higher rate than rod photoreceptors.
Aaland island eye disease (AIED): A retinal disease characterized by a combination of fundus hypopigmentation, decreased visual acuity due to foveal hypoplasia, nystagmus, astigmatism, protan color vision defect, myopia, and defective dark adaptation. Except for progression of axial myopia, the disease can be considered to be a stationary condition. Electroretinography reveals abnormalities in both photopic and scotopic functions.

Topology

Cytoplasmic: 1-92 aa
Helical: 93-111 aa
Extracellular: 112-129 aa
Helical: 130-149 aa
Cytoplasmic: 150-161 aa
Helical: 162-180 aa
Extracellular: 181-201 aa
Helical: 202-220 aa
Cytoplasmic: 221-239 aa
Helical: 240-259 aa
Extracellular: 260-347 aa
Helical: 348-372 aa
Cytoplasmic: 373-529 aa
Helical: 530-549 aa
Extracellular: 550-564 aa
Helical: 565-583 aa
Cytoplasmic: 584-591 aa
Helical: 592-610 aa
Extracellular: 611-620 aa
Helical: 621-639 aa
Cytoplasmic: 640-658 aa
Helical: 659-679 aa
Extracellular: 680-733 aa
Helical: 734-758 aa
Cytoplasmic: 759-871 aa
Helical: 872-890 aa
Extracellular: 891-906 aa
Helical: 907-926 aa
Cytoplasmic: 927-938 aa
Helical: 939-957 aa
Extracellular: 958-963 aa
Helical: 964-983 aa
Cytoplasmic: 984-1002 aa
Helical: 1003-1022 aa
Extracellular: 1023-1112 aa
Helical: 1113-1133 aa
Cytoplasmic: 1134-1190 aa
Helical: 1191-1209 aa
Extracellular: 1210-1224 aa
Helical: 1225-1244 aa
Cytoplasmic: 1245-1251 aa
Helical: 1252-1273 aa
Extracellular: 1274-1290 aa
Helical: 1291-1310 aa
Cytoplasmic: 1311-1329 aa
Helical: 1330-1349 aa
Extracellular: 1350-1416 aa
Helical: 1417-1441 aa
Cytoplasmic: 1442-1977 aa

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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