Mouse Anti-CHRNB3 Recombinant Antibody (3G6) (CBMAB-C6169-LY)

Name: Mouse Anti-CHRNB3 Recombinant Antibody (3G6)
SKU: CBMAB-C6169-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

3G6

Antibody Isotype

IgG2b, κ

Application

ELISA, IF, WB

Basic Information

Immunogen

CHRNB3 (128 a.a. ~ 223 a.a) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cholinergic Receptor Nicotinic Beta 3 Subunit

Introduction

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]

Entrez Gene ID

1142

UniProt ID

Q05901

Alternative Names

Cholinergic Receptor Nicotinic Beta 3 Subunit; Acetylcholine Receptor, Nicotinic, Beta 3 (Neuronal); Cholinergic Receptor, Nicotinic, Beta Polypeptide 3; Cholinergic Receptor, Nicotinic, Beta 3 (Neuronal); Cholinergic Receptor, Nicotinic Beta 3;

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Biological Process

Chemical synaptic transmission Source: GO_Central
Ion transmembrane transport Source: GO_Central
Nervous system process Source: GO_Central
Regulation of membrane potential Source: GO_Central
Response to nicotine Source: GO_Central
Signal transduction Source: GO_Central
Synaptic transmission, cholinergic Source: GO_Central

Cellular Location

Postsynaptic cell membrane; Cell membrane

Topology

Extracellular: 25-232
Helical: 233-257
Helical: 265-282
Helical: 299-320
Cytoplasmic: 321-428
Helical: 429-447

More Infomation

References

Shi, J., Chen, N., Wang, Z., Wang, F., Tan, Y., Tan, S., ... & Luo, X. (2021). Cholinergic receptor nicotinic beta 3 subunit polymorphisms and smoking in male Chinese patients with schizophrenia. EC psychology and psychiatry, 10(7), 11.

Drayson, L. E., & Triplett, J. W. (2019). A Chrnb3‐Cre BAC transgenic mouse line for manipulation of gene expression in retinal ganglion cells. genesis, 57(9), e23305.

Zhao, L., Zou, L. Y., Cheng, B. F., Yu, X. J., Zou, J. H., & Han, W. (2019). Chronic Obstructive Pulmonary Disease Risk and Smoking Cessation Changes Induced by CHRNA5-A3 and CHRNB3-A6 Variation in a Chinese Male Population. Balkan journal of medical genetics: BJMG, 22(2), 51.

Li, M. D. (2018). Contribution of Variants in CHRNB3/A6 Gene Cluster on Chromosome 8 to Smoking Dependence. In Tobacco Smoking Addiction: Epidemiology, Genetics, Mechanisms, and Treatment (pp. 71-93). Springer, Singapore.

Wen, L., Han, H., Liu, Q., Su, K., Yang, Z., Cui, W., ... & Li, M. D. (2017). Significant association of the CHRNB3-CHRNA6 gene cluster with nicotine dependence in the Chinese Han population. Scientific reports, 7(1), 1-10.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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