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Rabbit Anti-CKAP5 Recombinant Antibody (D2Z8J) (CBMAB-CP0320-LY)

The product is antibody recognizes CKAP5. The antibody D2Z8J immunoassay techniques such as: WB.
See all CKAP5 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D2Z8J
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala31 of human ch-TOG protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytoskeleton Associated Protein 5
Introduction
This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Entrez Gene ID
Human9793
Mouse75786
Rat311191
UniProt ID
HumanQ14008
MouseA2AGT5
RatF1M949
Alternative Names
Cytoskeleton Associated Protein 5; Colonic And Hepatic Tumor Overexpressed Gene Protein; Ch-TOG; Colonic And Hepatic Tumor Over-Expressed Gene Protein; Cytoskeleton-Associated Protein 5; EC 3.1.1.29; EC 2.7.7.6;
Function
Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825).

Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments (PubMed:27156448).
Biological Process
Cell division Source: UniProtKB-KW
Centrosome cycle Source: HGNC-UCL
Centrosome duplication Source: GO_Central
Ciliary basal body-plasma membrane docking Source: Reactome
Establishment or maintenance of microtubule cytoskeleton polarity Source: HGNC-UCL
G2/M transition of mitotic cell cycle Source: Reactome
Microtubule depolymerization Source: ARUK-UCL
Microtubule polymerization Source: GO_Central
Mitotic spindle organization Source: GO_Central
Positive regulation of microtubule nucleation Source: CACAO
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
RNA transport Source: HGNC-UCL
Spindle organization Source: UniProtKB
Cellular Location
Centrosome; Spindle pole; Spindle; Kinetochore. Detected on centrosomes and kinetochores during interphase and mitosis independently from TACC3 and clathrin. Located to spindle poles and microtubules during mitosis. In complex with TACC3 localized to microtubule plus-ends in mitosis and interphase. In complex with TACC3 and clathrin localized to inter-microtubule bridges in mitotic spindles. Accumulation sites at microtubule plus ends protruded approximately 100 nm from MAPRE1/EB1 sites in interphase cells.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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