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Mouse Anti-CKAP5 Recombinant Antibody (CKAP5) (CBMAB-CP0320-LY)

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Published Data

CBMAB-CP0320-LY-1

Mouse Anti-CKAP5 Recombinant Antibody (CKAP5) (CBMAB-CP0320-LY) in IF

RPE1 control and ch-TOG RNAi cells were treated with nocodazole to depolymerize microtubules. After washout and incubation in ice, microtubules were allowed to regrow for 10 s. Cells were fixed and stained with antibodies against GM130 (Golgi), α-tubulin (microtubules), and ch-TOG. Scale bar, 10 µm. ...View More

Ali, A., Vineethakumari, C., Lacasa, C., & Lüders, J. (2023). Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG. Nature Communications, 14(1), 289.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-CP0320-LY-2

Mouse Anti-CKAP5 Recombinant Antibody (CKAP5) (CBMAB-CP0320-LY) in WB

U2OS wild type cells or cells stably expressing recombinant GFP-tagged ch-TOG constructs were transfected with control or ch-TOG siRNA #1. Cell lysates were probed by western blotting for the indicated proteins. ...View More

Ali, A., Vineethakumari, C., Lacasa, C., & Lüders, J. (2023). Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG. Nature Communications, 14(1), 289.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-CP0320-LY-3

Mouse Anti-CKAP5 Recombinant Antibody (CKAP5) (CBMAB-CP0320-LY) in IF

Cells were analyzed by 3D-SIM for centrosomal localization of GFP-tagged ch-TOG constructs and of γ-tubulin. Illustrations indicate centriole orientations in the respective images. Scale bar, 1 μm. ...View More

Ali, A., Vineethakumari, C., Lacasa, C., & Lüders, J. (2023). Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG. Nature Communications, 14(1), 289.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-CP0320-LY-4

Mouse Anti-CKAP5 Recombinant Antibody (CKAP5) (CBMAB-CP0320-LY) in WB

Repression of ch-TOG (CKAP5) in cells with CDC45/GINS1 overexpression. A representative western blot shows siRNA mediated downregulation of ch-TOG (CKAP5) in the indicated stable cell lines. β-actin was used as loading control. ...View More

Böhly, N., Schmidt, A. K., Zhang, X., Slusarenko, B. O., Hennecke, M., Kschischo, M., & Bastians, H. (2022). Increased replication origin firing links replication stress to whole chromosomal instability in human cancer. Cell Reports, 41(11).

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-CP0320-LY-5

Mouse Anti-CKAP5 Recombinant Antibody (CKAP5) (CBMAB-CP0320-LY) in IP

CO-IP shows the binding of Annexin2, Annexin 5, Desmocollin1 and CEP290, exclusively with wtPOC5. Very weak interaction was observed between POC5A429 with Acetylated-α-tubulin, Galectin3 and 7, EHD4, CystatinA compared to wtPOC5. ...View More

Hassan, A., Parent, S., Mathieu, H., Zaouter, C., Molidperee, S., Bagu, E. T., ... & Moldovan, F. (2019). Adolescent idiopathic scoliosis associated POC5 mutation impairs cell cycle, cilia length and centrosome protein interactions. PLoS One, 14(3), e0213269.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CKAP5

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 1-300 mapping at the N-terminus of CKAP5 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cytoskeleton Associated Protein 5

Introduction

This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Entrez Gene ID

Human	9793
Mouse	75786
Rat	311191

UniProt ID

Human	Q14008
Mouse	A2AGT5
Rat	F1M949

Alternative Names

Cytoskeleton Associated Protein 5; Colonic And Hepatic Tumor Overexpressed Gene Protein; Ch-TOG; Colonic And Hepatic Tumor Over-Expressed Gene Protein; Cytoskeleton-Associated Protein 5; EC 3.1.1.29; EC 2.7.7.6;

Function

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825).

Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments (PubMed:27156448).

Biological Process

Cell division Source: UniProtKB-KW
Centrosome cycle Source: HGNC-UCL
Centrosome duplication Source: GO_Central
Ciliary basal body-plasma membrane docking Source: Reactome
Establishment or maintenance of microtubule cytoskeleton polarity Source: HGNC-UCL
G2/M transition of mitotic cell cycle Source: Reactome
Microtubule depolymerization Source: ARUK-UCL
Microtubule polymerization Source: GO_Central
Mitotic spindle organization Source: GO_Central
Positive regulation of microtubule nucleation Source: CACAO
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
RNA transport Source: HGNC-UCL
Spindle organization Source: UniProtKB

Cellular Location

Centrosome; Spindle pole; Spindle; Kinetochore. Detected on centrosomes and kinetochores during interphase and mitosis independently from TACC3 and clathrin. Located to spindle poles and microtubules during mitosis. In complex with TACC3 localized to microtubule plus-ends in mitosis and interphase. In complex with TACC3 and clathrin localized to inter-microtubule bridges in mitotic spindles. Accumulation sites at microtubule plus ends protruded approximately 100 nm from MAPRE1/EB1 sites in interphase cells.

More Infomation

References

Endo, Y., Takeda, K., Mohan, N., Shen, Y., Jiang, J., Rotstein, D., & Wu, W. J. (2018). Payload of T-DM1 binds to cell surface cytoskeleton-associated protein 5 to mediate cytotoxicity of hepatocytes. Oncotarget, 9(98), 37200.

Schneider, M. A., Christopoulos, P., Muley, T., Warth, A., Klingmueller, U., Thomas, M., ... & Meister, M. (2017). AURKA, DLGAP5, TPX2, KIF11 and CKAP5: Five specific mitosis-associated genes correlate with poor prognosis for non-small cell lung cancer patients. International journal of oncology, 50(2), 365-372.

Lu, A., Zhou, C. J., Wang, D. H., Han, Z., Kong, X. W., Ma, Y. Z., ... & Liang, C. G. (2017). Cytoskeleton-associated protein 5 and clathrin heavy chain binding regulates spindle assembly in mouse oocytes. Oncotarget, 8(11), 17491.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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