Mouse Anti-COL8A2 Recombinant Antibody (1F4) (V2LY-1206-LY705)

Name: Mouse Anti-COL8A2 Recombinant Antibody (1F4)
SKU: V2LY-1206-LY705
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat, Human

Clone

1F4

Antibody Isotype

IgG2a, κ

Application

WB, IP, ELISA

Basic Information

Immunogen

Amino acids 626-696 of COL8A2 of human.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

collagen, type VIII, alpha 2

Entrez Gene ID

Human	1296
Mouse	329941
Rat	313592

UniProt ID

Human	P25067
Mouse	P25318
Rat	D4ADG9

Function

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis (By similarity).

Biological Process

Angiogenesis Source: UniProtKB-KW
Camera-type eye morphogenesis Source: Ensembl
Cell-cell adhesion Source: UniProtKB
Collagen fibril organization Source: Reactome
Epithelial cell proliferation Source: Ensembl
Extracellular matrix organization Source: GO_Central

Cellular Location

Basement membrane

Involvement in disease

Corneal dystrophy, Fuchs endothelial, 1 (FECD1):
A corneal disease caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
Corneal dystrophy, posterior polymorphous, 2 (PPCD2):
A rare mild subtype of posterior corneal dystrophy characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit-lamp examination and specular microscopy. Affected patient typically are asymptomatic.

PTM

Proteolytically cleaved by neutrophil elastase, in vitro.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

More Infomation

References

Cheng, Y. X., Xiao, L., Yang, Y. L., Liu, X. D., Zhou, X. R., Bu, Z. F., ... & Wang, D. K. (2021). Collagen type VIII alpha 2 chain (COL8A2), an important component of the basement membrane of the corneal endothelium, facilitates the malignant development of glioblastoma cells via inducing EMT. Journal of Bioenergetics and Biomembranes, 53(1), 49-59.

Moschos, M. M., Diamantopoulou, A., Gouliopoulos, N., Droutsas, K., Bagli, E., Chatzistefanou, K., ... & Kroupis, C. (2019). TCF4 and COL8A2 gene polymorphism screening in a Greek population of late-onset Fuchs endothelial corneal dystrophy. in vivo, 33(3), 963-971.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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