Sign in or Register   Sign in or Register
  |  

Mouse Anti-EMC8 Recombinant Antibody (4F4) (CBMAB-A1801-LY)

The product is antibody recognizes COX4NB. The antibody 4F4 immunoassay techniques such as: WB, ELISA.
See all EMC8 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4F4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
COX4NB (NP_006058.1, 113 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ER Membrane Protein Complex Subunit 8
Entrez Gene ID
10328
UniProt ID
O43402
Alternative Names
C16orf2; C16orf4; FAM158B; NOC4
Research Area
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656).

Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231).

Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151).

It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231).

By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835).

By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
Biological Process
Protein insertion into ER membrane by stop-transfer membrane-anchor sequence Source: UniProtKB
Tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane

Iyer, A., Niemann, M., Serricchio, M., Dewar, C. E., Oeljeklaus, S., Farine, L., ... & Bütikofer, P. (2022). The endoplasmic reticulum membrane protein complex localizes to the mitochondrial-endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei. PLoS pathogens, 18(5), e1009717.

Guna, A., Hazu, M., Tomaleri, G. P., & Voorhees, R. M. (2022). A TAle of Two Pathways: Tail-Anchored Protein Insertion at the Endoplasmic Reticulum. Cold Spring Harbor Perspectives in Biology, a041252.

Bai, L., & Li, H. (2022). Cryo‐EM structures of the endoplasmic reticulum membrane complex. The FEBS Journal, 289(1), 102-112.

Alvira, S., Corey, R. A., Collinson, I., & Römisch, K. (2021). Membrane protein biogenesis by the EMC. The EMBO Journal, 40(2), e107407.

Yang, M., Li, S., Liu, W., Li, X., He, Y., Yang, Y., ... & Zhu, X. (2021). The ER membrane protein complex subunit Emc3 controls angiogenesis via the FZD4/WNT signaling axis. Science China Life Sciences, 64(11), 1868-1883.

Pleiner, T., Tomaleri, G. P., Januszyk, K., Inglis, A. J., Hazu, M., & Voorhees, R. M. (2020). Structural basis for membrane insertion by the human ER membrane protein complex. Science, 369(6502), 433-436.

Pleiner, T., Januszyk, K., Tomaleri, G. P., Oania, R. S., Hazu, M., Sweredoski, M. J., ... & Voorhees, R. M. (2020). Regulated assembly of the ER membrane protein complex. bioRxiv.

Volkmar, N., Thezenas, M. L., Louie, S. M., Juszkiewicz, S., Nomura, D. K., Hegde, R. S., ... & Christianson, J. C. (2019). The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis. Journal of Cell Science, 132(2), jcs223453.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-EMC8 Recombinant Antibody (4F4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare