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Mouse Anti-CYBA Recombinant Antibody (CBXC-0633) (V2LY-0125-LY849)


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Published Data
V2LY-0125-LY849-1
Mouse Anti-CYBA Recombinant Antibody (CBXC-0633) (V2LY-0125-LY849) in WB
Quantification of protein expression and representative images of p22phox as determined by Western Blot. ...View More
Barbu, E., Mihaila, A. C., Gan, A. M., Ciortan, L., Macarie, R. D., Tucureanu, M. M., ... & Butoi, E. (2024). The elevated inflammatory status of neutrophils is related to in-hospital complications in patients with acute coronary syndrome and has important prognosis value for diabetic patients. International Journal of Molecular Sciences, 25(10), 5107. Distributed under Open Access license CC BY 4.0, without modification.
V2LY-0125-LY849-2
Mouse Anti-CYBA Recombinant Antibody (CBXC-0633) (V2LY-0125-LY849) in WB
Overexpression of LSD1 triggers the up-regulation of Nox1, Nox2, Nox4, Nox5, and p22phox transcript levels in HEK293 reporter cells. ...View More
Manea, S. A., Vlad, M. L., Lazar, A. G., Muresian, H., Simionescu, M., & Manea, A. (2022). Pharmacological inhibition of lysine-specific demethylase 1A reduces atherosclerotic lesion formation in Apolipoprotein E-deficient mice by a mechanism involving decreased oxidative stress and inflammation; potential implications in human atherosclerosis. Antioxidants, 11(12), 2382. Distributed under Open Access license CC BY 4.0, without modification.
V2LY-0125-LY849-3
Mouse Anti-CYBA Recombinant Antibody (CBXC-0633) (V2LY-0125-LY849) in IF
Immunofluorescence stainings were repeated three times and different treatment groups were photographed with similar settings using a 40-fold objective (scale bar, 20 μm). Representative images from left to right panels are presenting p22phox (red), nucleus (blue), and merged image. ...View More
Korhonen, E., Hytti, M., Piippo, N., Kaarniranta, K., & Kauppinen, A. (2021). Antimycin A-induced mitochondrial dysfunction regulates inflammasome signaling in human retinal pigment epithelial cells. Experimental Eye Research, 209, 108687. Distributed under Open Access license CC BY 4.0, without modification.
V2LY-0125-LY849-4
Mouse Anti-CYBA Recombinant Antibody (CBXC-0633) (V2LY-0125-LY849) in WB
The protein expression of NADPH oxidase subunit p22phox (N = 6). ∗p < 0.05, vs. the Ctr group; #p < 0.05, vs. the DOCA group. ...View More
Cai, R., Hao, Y., Liu, Y. Y., Huang, L., Yao, Y., & Zhou, M. S. (2020). Tumor Necrosis Factor Alpha Deficiency Improves Endothelial Function and Cardiovascular Injury in Deoxycorticosterone Acetate/Salt-Hypertensive Mice. BioMed Research International, 2020(1), 3921074. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXC-0633
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-195 representing full length p22-phox of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome B-245 Alpha Chain
Entrez Gene ID
Human1535
Mouse13057
UniProt ID
HumanP13498
MouseQ61462
Function
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: Reactome
Cell redox homeostasis Source: Reactome
Cellular response to oxidative stress Source: Reactome
Cytochrome complex assembly Source: BHF-UCL
Hydrogen peroxide biosynthetic process Source: BHF-UCL
Inflammatory response Source: BHF-UCL
Innate immune response Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Positive regulation of cell killing Source: Reactome
Positive regulation of defense response to bacterium Source: UniProtKB
Positive regulation of interleukin-6 production Source: UniProtKB
Positive regulation of phagocytosis Source: UniProtKB
Positive regulation of reactive oxygen species biosynthetic process Source: UniProtKB
Positive regulation of toll-like receptor 2 signaling pathway Source: UniProtKB
Positive regulation of tumor necrosis factor production Source: UniProtKB
Respiratory burst Source: BHF-UCL
Smooth muscle hypertrophy Source: BHF-UCL
Superoxide anion generation Source: BHF-UCL
Superoxide metabolic process Source: BHF-UCL
Vascular endothelial growth factor receptor signaling pathway Source: Reactome
Cellular Location
Cell membrane. As unassembled monomer may localize to the endoplasmic reticulum.
Involvement in disease
Granulomatous disease, chronic, cytochrome-b-negative, autosomal recessive (ARCGD):A form of chronic granulomatous disease, a primary immunodeficiency characterized by severe recurrent bacterial and fungal infections, along with manifestations of chronic granulomatous inflammation. It results from an impaired ability of phagocytes to mount a burst of reactive oxygen species in response to pathogens.
PTM
The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.
Ubiquitinated at Lys-149 likely by RNF145.
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For research use only. Not intended for any clinical use.

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